Method of measuring blood component, sensor used in the method, and measuring device
First Claim
1. A method of measuring a component in blood, comprising:
- causing a redox reaction between the component in the blood and an oxidoreductase in the presence of a mediator;
detecting an oxidation current or a reduction current caused through the redox reaction by an electrode system; and
calculating an amount of the component based on a value of the detected current, wherein the method further comprises measuring a Hct value of the blood and correcting the amount of the component using this Hct value, the measurement of the Hct value comprising;
providing an electrode system having a working electrode and a counter electrode;
providing a mediator on the counter electrode but not on the working electrode;
supplying the blood to the electrode system;
applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the electrodes;
detecting the oxidation current or the reduction current; and
calculating the Hct value based on a value of the detected current.
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Abstract
The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The sensor for measuring a component in blood has a first analysis portion and a second analysis portion. The first analysis portion has a first electrode system (11,12) and a reagent layer (14), and the reagent layer (14) has an oxidoreductase that acts on the component and a mediator. In the first analysis portion, the component in the blood is measured by causing a redox reaction of the component with the oxidoreductase in the presence of the mediator and detecting a redox current caused when a voltage is applied by the first electrode (11,12). The second analysis portion has a working electrode and a counter electrode, and a mediator is provided on the counter electrode but not on the working electrode. In the second analysis portion, a Hct value of the blood is measured by supplying the blood to the electrode system, applying a voltage to cause a current to flow, and detecting a value of the current. Using this Hct value, the amount of the component is corrected.
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Citations
43 Claims
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1. A method of measuring a component in blood, comprising:
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causing a redox reaction between the component in the blood and an oxidoreductase in the presence of a mediator;
detecting an oxidation current or a reduction current caused through the redox reaction by an electrode system; and
calculating an amount of the component based on a value of the detected current, wherein the method further comprises measuring a Hct value of the blood and correcting the amount of the component using this Hct value, the measurement of the Hct value comprising;
providing an electrode system having a working electrode and a counter electrode;
providing a mediator on the counter electrode but not on the working electrode;
supplying the blood to the electrode system;
applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the electrodes;
detecting the oxidation current or the reduction current; and
calculating the Hct value based on a value of the detected current. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A sensor for measuring a component in blood by causing a redox reaction of the component and detecting an oxidation current or a reduction current caused through the redox reaction by an electrode,
wherein the sensor comprises: -
a first analysis portion comprising a first electrode system on which at least an oxidoreductase that acts upon the component and a mediator are provided; and
a second analysis portion comprising a second electrode system that comprises a working electrode and a counter electrode, a mediator being provided on the counter electrode but not on the working electrode, in the first analysis portion, the component in the blood is measured by causing a redox reaction between the component and the oxidoreductase in the presence of the mediator and detecting by the first electrode system an oxidation current or a reduction current caused to flow when a voltage is applied, and in the second analysis portion, a Hct value of the blood is measured by supplying the blood to the second electrode system, applying a voltage to the blood in this state to cause an oxidation current or a reduction current to flow between the working electrode and the counter electrode, and detecting a value of the oxidation current or the reduction current. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification