Bioinformatically detectable group of novel regulatory genes and uses thereof

• US 20070134655A1
• Filed: 11/16/2003
• Published: 06/14/2007
• Est. Priority Date: 11/14/2002
• Status: Active Grant

• Current Assignee: Rosetta Genomics Limited (Rosetta Genomics)
• Original Assignee: Rosetta Genomics Limited (Rosetta Genomics)
• Inventors: Bentwich, Itzhak
• Granted Patent: US 7,618,814 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: C12N 2330/10 naturally occurring
• CPC Class Codes: G16B 15/00 ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
• CPC Class Codes: G16B 15/10 Nucleic acid folding
• CPC Class Codes: G16B 20/00 ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
• CPC Class Codes: G16B 20/20 Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
• CPC Class Codes: G16B 20/30 Detection of binding sites or motifs

Abstract

The present invention relates to a first group of novel genes, here identified as genomic address messenger or GAM genes, and a second group of novel operon-like genes, here identified as genomic record or GR genes. GAM genes selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various diseases. Nucleic acid molecules are provided respectively encoding 8607 GAM genes, and 1096 GR genes, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM and GR genes and specific functions and utilities thereof, for detecting expression of GAM and GR genes, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof.

Claims

1. A bioinformatically detectable novel gene encoding substantially pure DNA wherein:

RNA encoded by said bioinformatically detectable novel gene is about 18 to about 24 nucleotides in length, and originates from an RNA precursor, which RNA precursor is about 50 to about 120 nucleotides in length;

a nucleotide sequence of a first half of said RNA precursor is a partial or accurate inversed reversed sequence of a nucleotide sequence of a second half thereof;

a nucleotide sequence of said RNA encoded by said novel gene is a partial or accurate inversed reversed sequence of a nucleotide sequence of a binding site associated with at least one target gene;

said novel gene cannot be detected by either of the following;

a visually discernable whole body phenotype; and

detection of 99.9% of RNA species shorter than 25 nucleotides expressed in a tissue sample; and

a function of said novel gene is bioinformatically deducible.

6. A bioinformatically detectable novel gene according to claim 1 and wherein said function of said novel gene is bioinformatically deducible from the following data elements:

said nucleotide sequence of said RNA encoded by said bioinformatically detectable novel gene,

a nucleotide sequence of said at least one target gene; and

a function of said at least one target gene.

7. A bioinformatically detectable novel gene according to claim 1 and wherein said RNA encoded by said novel gene complementarily binds said binding site associated with said at least one target gene, thereby modulating expression of said at least one target gene.

8. A bioinformatically detectable novel gene according to claim 1 and wherein:

said binding site associated with at least one target gene is located in an untranslated region of RNA encoded by said at least one target gene.

9. A bioinformatically detectable novel gene according to claim 7 and wherein:

said function of said novel gene is selective inhibition of translation of said at least one target gene, which selective inhibition comprises complementary hybridization of said RNA encoded by said novel gene to said binding site.

10. A vector comprising the DNA of claim 1

11. A method of selectively inhibiting translation of at least one gene, comprising introducing the vector of claim 10 into a cell.

12. A method according to claim 11 and wherein said introducing comprises utilizing RNAi pathway.

13. A gene expression inhibition system comprising:

the vector of claim 10 and

a vector inserter, functional to insert said vector of claim 10 into a cell, thereby selectively inhibiting translation of at least one gene.

14. A probe comprising the DNA of claim 1

15. A method of selectively detecting expression of at least one gene, comprising using the probe of claim 14

16. A gene expression detection system comprising:

the probe of claim 14;

and

a gene expression detector functional to selectively detect expression of at least one gene.

2. A bioinformatically detectable novel gene encoding substantially pure DNA wherein:

RNA encoded by said bioninformatically detectable novel gene comprises a plurality of RNA sections, each of said RNA sections being about 50 to about 120 nucleotides in length, and comprising an RNA segment, which RNA segment is about 18 to about 24 nucleotides in length;

a nucleotide sequence of a first half of each of said RNA sections encoded by said novel gene is a partial or accurate inversed reversed sequence of nucleotide sequence of a second half thereof;

a nucleotide sequence of each of said RNA segments encoded by said novel gene is a partial or accurate inversed reversed sequence of the nucleotide sequence of a binding site associated with at least one target gene; and

a function of said novel gene is bioinformatically deducible from the following data elements;

said nucleotide sequence of said RNA encoded by said novel gene, a nucleotide sequence of said at least one target gene, and function of said at least one target gene.

3. A bioinformatically detectable novel gene encoding substantially pure DNA wherein:

RNA encoded by said bioinformatically detectable novel gene is about 18 to about 24 nucleotides in length, and originates from an RNA precursor, which RNA precursor is about 50 to about 120 nucleotides in length;

a nucleotide sequence of a first half of said RNA precursor is a partial or accurate inversed reversed sequence of a nucleotide sequence of a second half thereof;

a nucleotide sequence of said RNA encoded by said novel gene is a partial or accurate inversed reversed sequence of a nucleotide sequence of a binding site associated with at least one target gene;

a function of said novel gene is modulation of expression of said at least one target gene; and

said at least one target gene does not encode a protein.

4. A bioinformatically detectable novel gene encoding substantially pure DNA wherein:

said bioinformatically detectable novel gene does not encode a protein;

RNA encoded by said bioinformatically detectable novel gene is maternally transferred by a cell to at least one daughter cell of said cell;

a function of said novel gene comprises modulation of a cell type of said daughter cell; and

said modulation is bioinformatically deducible.

5. A bioinformatically detectable novel gene encoding substantially pure DNA wherein:

said bioinformatically detectable novel gene does not encode a protein;

a function of said novel gene is promotion of expression of said at lease one target gene; and

said at least one target gene is bioinformatically deducible.