Device and method for multiple analyte detection
First Claim
1. A device comprising:
- a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
detection reagents pre-loaded in each of the two or more detection chambers;
wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, and the fluid connections branch off from opposite sides of the channel.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
82 Citations
46 Claims
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1. A device comprising:
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a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
detection reagents pre-loaded in each of the two or more detection chambers;
wherein the fluid communication comprises a channel and respective fluid connections connecting the channel to the respective detection chambers of the two or more detection chambers, and the fluid connections branch off from opposite sides of the channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A device comprising:
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a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
detection reagents pre-loaded in each of the two or more detection chambers;
wherein the fluid communication comprises an individual channel for each detection chamber of the two or more detection chambers, and each individual channel provides a connection between the inlet and the respective detection chamber and each individual channel communicates with only a single detection chamber. - View Dependent Claims (12, 13, 14)
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15. A device comprising:
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a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more detection chambers, and (iii) a fluid communication between the two or more detection chambers and the inlet; and
detection reagents pre-loaded in each of the two or more detection chambers;
wherein the substrate comprises two or more layers and at least one of the two or more layers comprises the two or more detection chambers formed therein and another of the two or more layers comprises the fluid communication formed therein. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. A device comprising:
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a substrate;
a sample-distribution network defined by the substrate and comprising (i) a sample inlet, (ii) two or more reaction chambers, and (iii) a fluid communication between the reaction chambers and the inlet; and
reagents pre-loaded in each of the two or more reaction chambers;
wherein the substrate comprises resistive traces for passing electrical current and that contact respective regions adjacent the reaction chambers. - View Dependent Claims (24, 25, 26, 27, 28)
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29. A method comprising
sealing a cover layer to a substrate to form a laminated substrate, the substrate comprising a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) a channel providing fluid connections between each of said two or more detection chambers and said inlet, wherein at least two of said two or more detection chambers each contains a different selected-sequence polynucleotide pre-loaded therein, and each selected-sequence polynucleotide is effective to selectively bind to a respective target polynucleotide.
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37. A method comprising
sealing a cover layer to a substrate to form a laminated substrate, the substrate comprising a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) a channel providing fluid connections between each of said two or more detection chambers and said inlet, wherein at least two of said two or more detection chambers each contains a different reagent adapted to detect a polypeptide, pre-loaded therein.
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42. A method comprising
sealing a cover layer to a substrate to form a laminated substrate, the substrate comprising a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) a channel providing fluid connections between each of said two or more detection chambers and said inlet, wherein at least two of said two or more detection chambers each contains a different reagent adapted to detect a polysaccharide.
Specification