Method for attachment of silylated molecules to glass surfaces
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Abstract
A method for the efficient immobilization of silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds, devices, and kits for modifying surfaces such as glass surfaces.
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Citations
131 Claims
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1-56. -56. (canceled)
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57. A device comprising a surface with an immobilized molecule, wherein said surface is prepared by the method comprising the steps of:
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(a) contacting Si(NCY)4 wherein Y represents oxygen or sulfur with an agent so as to form a first reactive intermediate, said agent having a formula ii;
(R1)(R2)(R3)Si—
X-Z
iiwherein R1, R2 and R3 independently represents C1-C6 alkoxy, C1-C6 alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C1-C6 alkyl and C1-C6 alkoxy;
X represents linear or branched C1-C20 alkyl or aryl substituted with one or more groups selected from the group consisting of C1-C6 alkyl and C1-C6 alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, or sulfur; and
Z represents a hydroxy or amino group, with the proviso that at least one of R1, R2 or R3 represents C1-C6 alkoxy;
(b) contacting the first reactive intermediate with a molecule so as to form a second reactive intermediate;
(c) contacting the second reactive intermediate with said surface so as to immobilized the molecule onto said surface. - View Dependent Claims (58, 59, 60, 61, 62, 63, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110)
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64-90. -90. (canceled)
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111. A device for immobilizing a molecule onto a glass surface, wherein the molecule is immobilized by the method comprising the steps of:
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(a) contacting Si(NCY)4 wherein Y represents oxygen or sulfur with an agent so as to form a first reactive intermediate, said agent having a formula ii;
(R1)(R2)(R3)Si—
X-Z
iiwherein R1, R2 and R3 independently represents C1-C6 alkoxy, C1-C6 alkyl, phenyl, or aryl substituted with one or more groups selected from the group consisting of C1-C6 alkyl and C1-C6 alkoxy;
X represents linear or branched C1-C20 alkyl or aryl substituted with one or more groups selected from the group consisting of C1-C6 alkyl and C1-C6 alkoxy, optionally substituted with one or more heteroatoms comprising oxygen, nitrogen, and sulfur; and
Z represents a hydroxy or amino group, with the provision that at least of one R1, R2, or R3 represents C1-C6 alkoxy;
(b) contacting the first reactive intermediate with a molecule so as to form a second reactive intermediate;
(c) contacting the second reactive intermediate with said surface so as to immobilized the molecule onto said surface. - View Dependent Claims (112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131)
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Specification