Screening method, screening program and screening device

• US 20070134730A1
• Filed: 12/13/2006
• Published: 06/14/2007
• Est. Priority Date: 12/13/2005
• Status: Abandoned Application

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Yamada, Takayuki, Ezoe, Toshihide, Tsuzuki, Hirohiko
• Application Number:
  US11/637,837
• Publication Number:
  US 20070134730A1
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: G01N 2500/04 Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
• CPC Class Codes: G01N 33/553 Metal or metal coated

Abstract

The invention provides a screening method for selecting a particular candidate molecule by using binding amount data between a target molecule and the candidate molecule, the data being obtained by supplying the candidate molecule of study to a measurement region composed of an immobilization membrane on which the target molecule is immobilized, the screening method comprising: obtaining the binding amount data during activity by supplying the candidate molecule to a measurement region in which the target molecule not subjected to activity lowering treatment that lowers physiological activity is immobilized, obtaining the binding amount data during low activity by supplying the candidate molecule to a measurement region in which the target molecule that is subjected to activity lowering treatment that lowers physiological activity is immobilized, and extracting a candidate molecule having a specified difference between the binding amount data during the activity and the binding amount data during the low activity.

Claims

1. A screening method for selecting a particular candidate molecule by use of data of binding amount between a target molecule and the candidate molecule, the data being obtained by supplying the candidate molecule of study to a measurement region composed of an immobilization membrane on which the target molecule is immobilized, the screening method comprising:

obtaining the data of binding amount during activity by supplying the candidate molecule to a measurement region in which the target molecule not subjected to activity lowering treatment that lowers physiological activity is immobilized,

obtaining the data of binding amount during low activity by supplying the candidate molecule to a measurement region in which the target molecule that is subjected to activity lowering treatment that lowers physiological activity is immobilized, and

extracting a candidate molecule having a specified difference between the data of binding amount during the activity and the data of binding amount during the low activity.

2. The screening method of claim 1, wherein

the extracting a candidate molecule includes extracting a candidate molecule showing data of binding amount during low activity of a threshold amount set in advance or less, or a candidate molecule showing data of binding amount during low activity of less than a threshold amount set in advance.

3. The screening method of claim 2, wherein

the extracting a candidate molecule includes extracting a candidate molecule showing data of binding amount during activity having a threshold amount set in advance or more, or a candidate molecule showing data of binding amount during activity exceeding a threshold amount set in advance.

4. The screening method of claim 1, further comprising:

carrying out activity lowering treatment on the target molecule subsequent to the obtaining of data of binding amount during activity.

5. The screening method of claim 1, wherein:

the activity lowering treatment on the target molecule is carried out after the target molecule is immobilized on an immobilization membrane.

6. The screening method of claims 1, wherein:

the immobilization membrane is formed on one side of a metal membrane and not formed on the other side, and

the obtaining data of binding amount during activity and the obtaining data of binding amount during low activity are carried out by a process including using attenuated total reflection generated by irradiating a light beam to the opposite side of the metal membrane to that on which the immobilization membrane is formed.

7. The screening method of claim 6, wherein:

obtaining the data of binding amount during activity and obtaining the data of binding amount during low activity are carried out by a process including supplying the candidate molecule to the metal membrane on which the immobilization membrane is formed on which the target molecule is not immobilized, and by setting as a reference data the amount of attenuated total reflection generated by irradiating the light beam to the opposite side of the metal membrane to that on which the immobilization membrane on which the target molecule is not immobilized is formed.

8. A screening program for selecting a particular candidate molecule by use of data of binding amount between a target molecule and a candidate molecule, the data being obtained by supplying the candidate molecule of study to a measurement region composed of an immobilization membrane on which the target molecule is immobilized, the screening program causing a computer to execute operations, the screening program operations comprising:

calculating data of binding amount during activity, based on binding amount between the target molecule and the candidate molecule supplied to a measurement region in which the target molecule not subjected to activity lowering treatment that lowers physiological activity is immobilized,

calculating data of binding amount during low activity, based on binding amount between the target molecule and the candidate molecule supplied to a measurement region in which the target molecule that is subjected to activity lowering treatment that lowers physiological activity is immobilized, and

extracting a candidate molecule showing a specified difference between the data of binding amount during the activity and the data of binding amount during the low activity.

9. The screening program of claim 8, further comprising:

executing activity lowering treatment of supplying to the target molecule an activity lowering treatment solution for lowering the physiological activity of the target molecule after calculating the data of binding amount during activity, and

instructing proceeding to the calculating the data of binding amount during the low activity after the completion of the activity lowering treatment.

10. The screening program of claim 8, wherein

the extracting includes comparing a threshold amount that has been input for selecting a candidate molecule using the data of binding amount during the low activity with data of binding amount during low activity, and extracting a candidate molecule showing data of binding amount during low activity of the threshold amount or less, or extracting a candidate molecule showing data of binding amount during low activity of less than the threshold amount.

11. The screening program of claim 9, wherein

the extracting includes comparing a threshold amount that has been input for selecting a candidate molecule using the data of binding amount during the low activity with data of binding amount during low activity, and extracting a candidate molecule showing data of binding amount during low activity of the threshold amount or less, or extracting a candidate molecule showing data of binding amount during low activity of less than the threshold amount.

12. The screening program of claim 10, wherein

the extracting includes comparing a threshold amount input for selecting a candidate molecule using the data of binding amount during activity with data of binding amount during activity, and extracting a candidate molecule showing the data of binding amount during activity of the threshold amount or more, or extracting a candidate molecule showing the data of binding amount during activity exceeding the threshold amount.

13. The screening program of claim 11, wherein

the extracting includes comparing a threshold amount input for selecting a candidate molecule using the data of binding amount during activity with data of binding amount during activity, and extracting a candidate molecule showing the data of binding amount during activity of the threshold amount or more, or extracting a candidate molecule showing the data of binding amount during activity exceeding the threshold amount.

14. The screening program of claims 8, wherein:

the immobilization membrane is formed on one side of a metal membrane and not formed on the other side, and

obtaining the data of binding amount during the activity and obtaining the data of binding amount during the low activity is carried out by a process including using attenuated total reflection generated by irradiating a light beam to the opposite side of the metal membrane to that on which the immobilization membrane is formed.

15. The screening program of claim 14, wherein:

obtaining the data of binding amount during activity and obtaining the data of binding amount during low activity are carried out by a process including supplying the candidate molecule to the metal membrane on which the immobilization membrane is formed on which the target molecule is not immobilized, and based on reference data obtained from the amount of attenuated total reflection generated by irradiating the light beam to the opposite side of the metal membrane to that on which the immobilization membrane on which the target molecule is not immobilized is formed.

16. A screening device for selecting a particular candidate molecule by use of data of binding amount between a target molecule and a candidate molecule, the data being obtained by supplying the candidate molecule of study to a measurement region composed of an immobilization membrane on which the target molecule is immobilized, the screening device comprising:

a measuring unit that obtains the data of binding amount by supplying a candidate molecule to the measurement region;

an activity lowering treatment mechanism that lowers the physiological activity of the target molecule by supplying an activity lowering treatment solution to the measurement region;

a memory that stores for every candidate molecule the data that is obtained from the measuring unit of the active binding amount of the candidate molecule to the target molecule not subjected to the activity lowering treatment, and stores for every candidate molecule data that is obtained from the measuring unit at low activity of the binding amount of an candidate molecule to a low activity target molecule after the activity lowering treatment; and

extracting unit that extracts a candidate molecule having a specified difference between the data of active binding amount stored in the memory and the low activity measurement data.

17. The screening device of claim 16, wherein

the extracting unit extracts a candidate molecule showing data of binding amount during low activity of a threshold that has been input or less, or extracts a candidate molecule showing data of binding amount during low activity of less than the threshold that has been input.

18. The screening device of claim 17, wherein

the extracting unit extracts a candidate molecule showing data of binding amount during activity of a threshold that has been input or more, or extracts a candidate molecule showing data of binding amount during activity exceeding the threshold.

19. The screening device of claims 16, wherein:

the immobilization membrane is formed on one side of a metal membrane and is not formed on the other side, and

obtaining the data of binding amount during activity and obtaining the data of binding amount during low activity are carried out by a process including using attenuated total reflection generated by irradiating a light beam to the opposite side of the metal membrane to that on which the immobilization membrane is formed.

20. The screening device of claim 19, wherein:

obtaining the data of binding amount during activity and obtaining the data of binding amount during low activity are carried out by a process including supplying the candidate molecule to a metal membrane on which is formed the immobilization membrane on which the target molecule is not immobilized, the data being based on reference data obtained from the amount of attenuated total reflection generated by irradiating the light beam to the opposite side of the metal membrane to that on which the immobilization membrane on which the target molecule is not immobilized is formed.