Method, apparatus and system for characterizing pathological specimen

• US 20070135999A1
• Filed: 07/05/2006
• Published: 06/14/2007
• Est. Priority Date: 12/13/2005
• Status: Abandoned Application

• Current Assignee: Applied Spectral Imaging Ltd.
• Original Assignee: Applied Spectral Imaging Ltd.
• Inventors: Kolatt, Tsafrir
• Application Number:
  US11/480,480
• Publication Number:
  US 20070135999A1
• Time in Patent Office: Days
• US Class Current: 702/19
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1475 using image analysis for extracting features of the particle
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 33/5082 Supracellular entities, e.g. tissue, organisms
• CPC Class Codes: G06T 2207/30072 Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06V 20/695 Preprocessing, e.g. image segmentation

Abstract

A method for characterizing a stained pathological specimen is disclosed. The method comprises obtaining an image of the specimen, classifying the picture-elements of the image into classification groups, and using the classification groups to define at least one set of picture-elements corresponding to at least one tissue region of the pathological specimen. The method further comprises applying, on each set of picture-elements, at least one set-operator so as to characterize the tissue regions according to image data and spatial characteristics of the set.

Claims

1. A method of analyzing an image of a stained pathological specimen, the image being arranged gridwise in a plurality of picture-elements, each being associated with image data over a grid, the method comprising:

defining at least one set of picture-elements over said grid, and applying, on each set of picture-elements, at least one set-operator, wherein each set-operator is associated with a predetermined diagnosis describing the pathological specimen, thereby analyzing the image.

2. The method of claim 1, further comprising issuing a report describing the pathological specimen, based on results obtained by said application of said at least one set-operator.

3. The method of claim 1, wherein said at least one set-operator comprises an operator for calculating statistical distributions.

4. The method of claim 1, wherein said at least one set-operator comprises an operator for calculating statistical moments.

5. The method of claim 1, wherein said at least one set-operator comprises an operator for calculating tensor of inertia.

6. The method of claim 1, wherein said at least one set-operator comprises an operator for calculating coordinates.

7. The method of claim 1, wherein said at least one set-operator comprises an operator for calculating population characteristics of said at least one set of picture-elements, hence to provide a population map characterizing the stained pathological specimen.

8. A method of characterizing a stained pathological specimen, the method comprising:

obtaining an image of the specimen, said image being arranged gridwise in a plurality of picture-elements each being associated with image data;

classifying said picture-elements into classification groups according to said image data;

using said classification groups to define at least one set of picture-elements corresponding to at least one tissue region of the pathological specimen; and

applying, on each set of picture-elements, at least one set-operator so as to characterize said tissue regions according to image data and spatial characteristics of said set;

thereby characterizing the pathological specimen.

9. The method of claim 8, further comprising using said classification groups to define at least one set of picture-elements corresponding to at least one background region of the pathological specimen.

10. The method of claim 8, wherein said defining said at least one set of picture-elements comprises clustering at least a portion of said picture-elements according to said classification groups, thereby providing at least one cluster of picture-elements.

11. The method of claim 10, wherein said applying said at least one set-operator on each said set of picture-elements, comprises applying said at least one set-operator on said at least one cluster of picture-elements.

12. The method of claim 8, wherein said defining said at least one set of picture-elements comprises applying a geometrical modeling procedure to at least a portion of said plurality of picture-elements.

13. The method of claim 10, wherein said defining said at least one set of picture-elements comprises applying a geometrical modeling procedure to said at least one cluster of picture-elements.

14. The method of claim 8, further comprising normalizing said image data prior to said classification.

15. The method of claim 8, further comprising combining at least a portion of said classification groups.

16. The method of claim 8, further comprising employing at least one counting technique to the stained pathological specimen, and correlating the results of said counting technique with said at least one set of picture-elements.

17. Apparatus for characterizing a stained pathological specimen based on an image of the specimen, the image being arranged in a plurality of picture-elements each being associated with image data, the apparatus comprising:

classification unit, for classifying said picture-elements into classification groups according to said image data;

a set definition unit, for defining at least one set of picture-elements corresponding to at least one tissue region of the pathological specimen, using said classification groups;

a data analysis unit, for applying at least one set-operator on each set of picture-elements, so as to characterize said tissue regions according to image data and spatial characteristics of said set, thereby characterizing the pathological specimen.

18. A system for characterizing a stained pathological specimen, comprising an imaging apparatus, for providing the image of the specimen, and the apparatus of claim 17.

19. The apparatus of claim 17, wherein said set definition unit is operable to define at least one set of picture-elements corresponding to at least one background region of the pathological specimen, using said classification groups.

20. The apparatus of claim 17, further comprising a clustering unit, for clustering at least a portion of said picture-elements according to said classification groups, to provide at least one cluster of picture-elements.

21. The apparatus of claim 20, wherein said data analysis unit is operable to apply said at least one set-operator on said at least one cluster of picture-elements.

22. The apparatus of claim 17, further comprising a geometrical modeling unit for applying a geometrical modeling procedure to at least a portion of said plurality of picture-elements.

23. The apparatus of claim 20, further comprising a geometrical modeling unit for applying a geometrical modeling procedure to said at least one cluster of picture-elements.

24. The apparatus of claim 17, wherein said classification unit is operable to normalize said image data.

25. The apparatus of claim 17, wherein said classification unit is operable to combine at least a portion of said classification groups.

26. The apparatus of claim 20, wherein said at least one cluster comprises at least one sub-cluster.

27. The apparatus of claim 26, wherein said geometrical modeling is applied to said at least one sub-cluster.

28. The apparatus of claim 20, wherein said at least one cluster comprises at least one cluster of background picture-elements.

29. The apparatus of claim 28, wherein said at least one set of picture-elements is defined by cross correlation of at least one cluster of background picture-elements with at least one cluster of non-background picture-elements.

30. The apparatus of claim 17, wherein said image comprises a spectral image and said image data comprises a wavelength spectrum.

31. The apparatus of claim 17, wherein said image comprises a monochrome image and said image data comprises intensity values.

32. The apparatus of claim 30, wherein said classification groups are selected from a predefined set of classification groups, each being associated with a predetermined wavelength spectrum.

33. The apparatus of claim 30, wherein said classification groups are defined based on the wavelength spectra of said picture-elements.

34. The apparatus of claim 33, wherein said classification groups are defined iteratively.

35. The apparatus of claim 33, wherein said classification groups are defined non-iteratively.

36. The apparatus of claim 17, wherein said at least one set-operator comprises an operator for calculating statistical distributions.

37. The apparatus of claim 17, wherein said at least one set-operator comprises an operator for calculating statistical moments.

38. The apparatus of claim 17, wherein said at least one set-operator comprises an operator for calculating tensor of inertia.

39. The apparatus of claim 22, wherein said at least one set-operator comprises an operator for calculating distribution of parameters obtained from said geometrical modeling procedure.

40. The apparatus of claim 17, wherein said at least one set-operator comprises an operator for calculating coordinates.

41. The apparatus of claim 20, wherein said at least one set-operator comprises an operator for calculating an average normalization factor over said at least one cluster of picture-element.

42. The apparatus of claim 17, wherein said at least one set-operator comprises an operator for calculating population characteristics of said at least one set of picture-elements, hence to provide a population map characterizing the stained pathological specimen.

43. The method of claim 42, further comprising employing at least one counting technique to the stained pathological specimen, to provide an amplification map characterizing the stained pathological specimen, and correlating said amplification map with said population map.

44. The apparatus of claim 30, wherein said spectral image is characterized by two spatial dimensions.

45. The apparatus of claim 30, wherein said spectral image is characterized by three spatial dimensions.

46. The apparatus of claim 17, wherein said image comprises a set of spectral images and said image data comprises a wavelength spectrum.

47. The apparatus of claim 46, wherein at least two images of said set of spectral images are characterized by a different magnification level.

48. The apparatus of claim 46, wherein at least two images of said set of spectral images are captured following a different staining of the pathological specimen.

49. The apparatus of claim 46, wherein at least two images of said set of spectral images are captured by a different illumination scheme.

50. The apparatus of claim 46, wherein at least two images of said set of spectral images are captured by a different spectral acquisition scheme.

51. The apparatus of claim 46, wherein at least two images of said set of spectral images correspond to different region-of-interests of the pathological specimen.

52. The apparatus of claim 17, wherein the pathological image is stained with a stain selected from the group consisting of a direct immunohistochemical stain, a secondary immunohistochemical stain, a histological stain, immunofluorescence stain, a DNA ploidy stain, a nucleic acid sequence specific probe and any combination thereof.

53. The apparatus of claim 17, wherein the pathological image is stained using a method selected from the group consisting of Romanowsky-Giemsa staining, Haematoxylin-Eosin staining and May-Grunwald-Giemsa staining.