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Fusion proteins useful for detecting analytes

  • US 20070136825A1
  • Filed: 09/13/2002
  • Published: 06/14/2007
  • Est. Priority Date: 09/18/2001
  • Status: Abandoned Application
First Claim
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1. A fusion protein comprising:

  • (a) two detection portions, wherein (i) the first detection portion is an energy-emitting protein portion and the second detection portion is a fluorescent protein portion;

    or (ii) the two detection portions are portions of a split fluorescent protein; and

    (b) a periplasmic binding protein (PBP) portion between said two detection portions, which undergoes a conformational change upon binding of a compound;

    wherein said conformational change results in a change of the energy emitted by said two detection portions.

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