Methods and systems for molecular fingerprinting
First Claim
1. A molecular fingerprinting method comprising the steps of:
- (a) identifying a target polynucleotide;
(b) selecting at least one fragment of the target polynucleotide, wherein the fragment is a fixed distance from a restriction site, to generate a set of one or more polynucleotide fragments; and
(c) designating some or all of the set of fragments as molecular fingerprint corresponding to the target polynucleotide.
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Abstract
This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacterial typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments.
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Citations
16 Claims
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1. A molecular fingerprinting method comprising the steps of:
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(a) identifying a target polynucleotide;
(b) selecting at least one fragment of the target polynucleotide, wherein the fragment is a fixed distance from a restriction site, to generate a set of one or more polynucleotide fragments; and
(c) designating some or all of the set of fragments as molecular fingerprint corresponding to the target polynucleotide. - View Dependent Claims (2, 3)
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4. A method for identifying a polynucleotide sample comprising the steps of:
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(a) identifying a target polynucleotide;
(b) selecting at least one fragment of the target polynucleotide, wherein the fragment is a fixed distance from a restriction site, to generate a set of one or more polynucleotide fragments;
(c) designating some or all of the set of fragments as a fingerprint corresponding to the target polynucleotide;
(d) synthesizing one or more oligonucleotide probes to complement the set of polynucleotide fragments;
(e) combining the probes, a polynucleotide sample, nucleotide triphosphates, and polymerase to synthesize at least one polynucleotide strand;
(f) cutting the strands with restriction enzymes to yield a set of sample fragments of fixed length; and
(g) comparing the set of sample fragments to the fingerprint. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16-55. -55. (canceled)
Specification