Polyethylene binding peptides and methods of use

• US 20070141628A1
• Filed: 12/01/2006
• Published: 06/21/2007
• Est. Priority Date: 12/15/2005
• Status: Active Grant

• Current Assignee: DuPont Safety & Construction, Inc. (DuPont de Nemours, Inc.)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Wang, Hong, O'Brien, John, Cunningham, Scott, Lowe, David, Wilkins, Antoinette
• Granted Patent: US 7,906,617 B2
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: C07K 14/001 by chemical synthesis
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393

Abstract

Combinatorially generated peptides are provided that have binding affinity for polyethylene (PE). The peptides may be used to deliver benefit agents to various PE surfaces.

Claims

1. A peptide reagent having a general structure selected from the group consisting of:

PE_m−

(PEBP)_n;

 

 

a)
PE_m−

(PEBP−

BAp)n;

 

 

b)
PE_m−

(PEBP−

AD)_n;

 

 

c)
PE_m−

(PEBP−

TBD)_n; and

 

 

d)
PE_m−

(PEBP−

L−

BA)n; and

 

 

e)
PE_m−

[(PEBP)_q−

L(x)−

(PEBP)r]n−

L−

BA;

 

 

f)

wherein;

i) PE is a PE moiety

ii) PEBP is a PE binding peptide having a PE binding domain;

iii) BA is at least one benefit agent;

iv) AD is at least one active domain incorporated into a PE binding peptide;

v) TBD is at least one target binding domain incorporated into a PE binding peptide;

vi) L is a linker molecule;

vii) m=the number of PE moieties available for binding;

viii) n=is less than or equal to m;

xi) p=1-20;

x) x=1-20; and

xi) r=1-50.

3. A peptide reagent according to either of claims 1 or 2 wherein the PE binding peptide comprises a PE binding sequence selected from the group consisting of SEQ ID NO'"'"'s:

1-7.

4. A peptide reagent according to claim 1 or 2 wherein the linker molecule is selected from the group consisting of:

a peptide linker, and an organic linker.

5. A peptide reagent according to claim 1 or 2 wherein the active domain has a functionality selected from the group consisting of:

a linker, a binding functionality, a catalytic functionality and an antimicrobial functionality.

6. A peptide reagent according to claim 5 wherein the active domain is a linker domain having the amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

176-179.

7. A peptide reagent according to claim 1 wherein the target binding domain has an affinity selected from the group consisting of affinity for pigments, affinity for benefit agents, affinity for print media, for affinity chemical functional groups, affinity for body surfaces, and affinity for biological analytes.

8. A peptide reagent according to either claim 7 wherein the target binding peptide binds to a target selected from the group consisting of;

proteins, nucleic acids, cells, cell membrane fractions, antibodies, antibody fragments, viral proteins, plant fibers, synthetic fibers, and organic and inorganic complexes.

9. A peptide reagent according to claim 7 wherein the target binding domain is a body surface binding domain.

10. A peptide reagent according to claim 9 wherein the body surface binding domain binds to a body surface selected from the group consisting of hair, skin, nails and teeth.

11. A peptide reagent according to claim 10 wherein the body surface binding domain binding hair has an amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

88-175.

12. A peptide reagent according to claim 10 wherein the body surface binding domain binding skin has an amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

80-87

13. A peptide reagent according to claim 10 wherein the body surface binding domain binding nails has an amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

88 and 89.

14. A peptide reagent according to claim 7 wherein the target binding domain is a print media binding domain.

15. A peptide reagent according to claim 14 wherein the print media binding domain has the amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

67-79.

16. A peptide reagent according to claim 7 wherein the target binding domain comprises a pigment binding domain.

17. A peptide reagent according to claim 16 wherein the pigment binding domain has the amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

42-66.

18. A peptide reagent according to claim 5 wherein the active domain comprises an animicrobial domain.

19. A peptide reagent according to claim 18 wherein the antimicrobial domain has an amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

13-41.

20. A peptide reagent according to either claim 1 or 2 wherein the PE moiety is incorporated into a surface.

21. A peptide reagent according to claim 20 wherein the surface is selected from the group consisting of a solid support, a bead, a microsphere, a sheet, and a fiber.

22. A peptide reagent according to claim 21 wherein the microsphere comprises a dye.

23. A peptide reagent according to claim 20 wherein the surface is coated on secondary surface.

24. A peptide reagent according to either claim 1 or 2 wherein the PE moiety is comprised within a PE film.

25. A peptide reagent according to either of either claim 1 or 2 wherein the PE moiety is comprised within a print medium.

26. A peptide reagent according to claim 25 wherein the print medium is selected from the group consisting of paper, sheets, films, nonwovens and textile fabrics.

27. A peptide reagent according to either claim 1 or 2 wherein the benefit agent is selected from the group consisting of;

pharmaceuticals, markers, colorants, conditioners and fragrances.

28. A peptide reagent according claim 27 wherein the pharmaceutical is selected from the group consisting of antibacterial agents, antiviral agents, antifungal agents, anti-cancer agents, vaccines, radiolabels, anti-inflammatories, anti-glaucomic agents, anesthetics, anti-neoplastic agents, antibodies, and hormones.

29. A peptide reagent according claim 27 wherein the marker is selected from the group consisting of:

a fluorescent label and an enzyme.

30. A peptide reagent according claim 27 wherein the conditioner is a hair conditioner selected from the group consisting of cationic polymers;

cationic surfactants, fatty alcohols, fatty amines;

waxes;

esters;

nonionic polymers, silicones;

siloxanes;

polymer emulsions; and

volumizing agents.

31. A peptide reagent according claim 27 wherein the conditioner is a skin conditioner selected from the group consisting of alpha-hydroxy acids, beta-hydroxy acids, polyols, hyaluronic acid, D,L-panthenol, polysalicylates, vitamin A palmitate, vitamin E acetate, glycerin, sorbitol, silicones, silicone derivatives, lanolin, natural oils and triglyceride esters.

32. A peptide reagent according claim 27 wherein the colorant is a hair colorant selected from the group consisting of D&

C Yellow 1 and 3, HC Yellow 6 and 8, D&

C Blue 1, HC Blue 1, HC Brown 2, HC Red 5, 2-nitro-paraphenylenediamine, N,N-hydroxyethyl-2-nitro-phenylenediamine, 4-nitro-indole, iron oxides, and carbon black.

33. A peptide reagent according claim 27 wherein the colorant is a skin colorant selected from the group consisting of D&

C Red No. 21;

D&

C Red No. 27, D&

C Red Orange No. 5;

D&

C Orange No. 10, titanium dioxide, zinc oxide, D&

C Red No. 36;

D&

C Orange No. 17, the calcium lakes of D&

C Red Nos. 7, 11, 31 and 34, the barium lake of D&

C Red No. 12, the strontium lake D&

C Red No. 13, the aluminum lakes of FD&

C Yellow No. 5, of FD&

C Yellow No. 6, of D&

C Red No. 27, of D&

C Red No. 21, of FD&

C Blue No. 1, iron oxides, manganese violet, chromium oxide, ultramarine blue, and carbon black.

34. A peptide reagent according claim 27 wherein the colorant is a nail colorant selected from the group consisting of D&

C Red Nos. 8, 10, 30 and 36, the barium lakes of D&

C Red Nos. 6, 9 and 12, the calcium lakes of D&

C Red Nos. 7, 11, 31 and 34, the strontium lake of D&

C Red No. 30 and D&

C Orange No.17 and D&

C Blue No. 6.

35. A method for binding a substrate comprising PE to a target comprising:

a) providing the peptide reagent according to claim 1;

and

b) contacting the peptide reagent of (a) with a substrate comprising a PE moiety under conditions whereby the peptide reagent binds to the PE moiety.

36. A method for delivering a benefit agent to a substrate comprising PE comprising:

a) providing the peptide reagent according to claim 1 having a benefit agent; and

b) contacting the peptide reagent of (a) with a substrate comprising a PE moiety under conditions whereby the peptide reagent binds to the PE moiety, whereby the benefit agent is delivered to the substrate.

37. A method according to claim 36 wherein the benefit agent is selected from the group consisting of pharmaceuticals, markers, colorants, conditioners and fragrances.

40. A peptide reagent according to either of claims 1 or 2 wherein the PE binding peptide is isolated by a process comprising the steps of:

(i) providing a library of combinatorially generated peptides;

(ii) contacting the library of (i) with a PE sample to form a reaction solution comprising;

(A) peptide-PE complex;

(B) unbound PE, and

(C) uncomplexed peptides;

(iii) isolating the peptide-PE complex of (ii);

(iv) eluting the weakly bound peptides from the isolated peptide complex of (iii) whereby the PE binding peptide is isolated.

2. A peptide reagent having the general structure:

(BA)_n−

L_m−

PE_p−

[(X)_a—

(Y)_b]_q−

L_r−

(BA)_s

Wherein;

i) BA is a benefit agent;

ii) PE is a PE moiety;

iii) L is a linker molecule;

iv) X is a PE peptide binding domain;

v) Y is an active domain; and

vi) wherein a, b, m, n, p, q, r, and s are non-negative integers wherein, b, n, r, and s may be 0; and

a, p and q will at least be 1.

38. A method for adhering two surfaces comprising:

a) providing a first surface comprising PE comprising a first peptide regent having the general formula;

(PEBP−

AD1)

wherein;

i) PmPB is a PE binding peptide; and

ii) AD1 is a first active domain;

b) providing a second surface comprising a target molecule comprising a second peptide reagent have the general formula;

(TBP−

AD2)

wherein;

iii) TBP is a target binding peptide; and

iv) AD2 is a second active domain having affinity for the first active domain;

c) juxtaposing the first and second surfaces wherein the first and second peptide reagents adhere to each other through the first and second active domains, whereby the surfaces are adhered.

39. A method for adhering two surfaces comprising:

a) providing a first surface comprising a first target molecule comprising a first peptide regent having the general formula;

(TBP1−

AD)

wherein;

i) TBP1 is a first target binding peptide; and

ii) AD is an active domain having binding affinity for PE;

b) providing a second surface comprising a second target molecule comprising a second peptide reagent have the general formula;

(TBP2−

AD)

wherein;

iii) TBP2 is a second target binding peptide; and

iv) AD is an active domain having binding affinity for PE;

c) juxtaposing the first and second surfaces in the presence of a PE moiety wherein the first and second peptide reagents adhere to the PE moiety through the active domain, whereby the surfaces are adhered.

41. A PE binding peptide having an amino acid sequence selected from the group consisting of SEQ ID NO'"'"'s:

1-7.