Method and agents for improving plant productivity involving endophytic actinomycetes and metabolites thereof
First Claim
1. A method of improving plant productivity said method comprising introducing into said plant or propagation material thereof:
- (i) an effective number of endophytic actinomycetes or variants, mutants or homologues thereof, which actinomycetes facilitate induction of at least one characteristic related to improved productivity; and
/or (ii) an effective amount of one or more metabolites derived from the actinomycetes of (i) or derivative, homologue, analogue, chemical equivalent or mimetic thereof;
for a time and under conditions sufficient to induce, in the subject plant, said characteristic, and wherein said actinomycete is selected from;
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
1 or a nucleotide sequence capable of hybridising to <
400>
1 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (b) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
2 or a nucleotide sequence capable of hybridising to <
400>
2 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
7 or a nucleotide sequence capable of hybridising to <
400>
7 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (d) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
10 or a nucleotide sequence capable of hybridising to <
400>
10 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
12 or a nucleotide sequence capable of hybridising to <
400>
12 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (f) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
13 or a nucleotide sequence capable of hybridising to <
400>
13 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
16 or a nucleotide sequence capable of hybridising to <
400>
16 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. (h) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
18 or a nucleotide sequence capable of hybridising to <
400>
18 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete (i) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
24 or a nucleotide sequence capable of hybridising to <
400>
24 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.
1 Assignment
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Accused Products
Abstract
The present invention relates to a method for improving plant productivity, and in particular crop yields, via the introduction of an endophytic microorganism to the subject plant. More particularly, the present invention is directed to a method for improving cereal crop productivity via the introduction of an endophytic actinomycete to the subject crop. The method of the present invention facilitates the improvement of crop productivity, such as increasing germination, by, inter alia, providing the subject plant with disease bio-control capabilities and up-regulating plant growth promoting activities. The present invention is also directed to novel endophytic microorganisms and uses thereof.
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Citations
45 Claims
-
1. A method of improving plant productivity said method comprising introducing into said plant or propagation material thereof:
-
(i) an effective number of endophytic actinomycetes or variants, mutants or homologues thereof, which actinomycetes facilitate induction of at least one characteristic related to improved productivity; and
/or(ii) an effective amount of one or more metabolites derived from the actinomycetes of (i) or derivative, homologue, analogue, chemical equivalent or mimetic thereof;
for a time and under conditions sufficient to induce, in the subject plant, said characteristic, and wherein said actinomycete is selected from;
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
1 or a nucleotide sequence capable of hybridising to <
400>
1 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(b) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
2 or a nucleotide sequence capable of hybridising to <
400>
2 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
7 or a nucleotide sequence capable of hybridising to <
400>
7 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(d) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
10 or a nucleotide sequence capable of hybridising to <
400>
10 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
12 or a nucleotide sequence capable of hybridising to <
400>
12 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(f) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
13 or a nucleotide sequence capable of hybridising to <
400>
13 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
16 or a nucleotide sequence capable of hybridising to <
400>
16 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(h) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
18 or a nucleotide sequence capable of hybridising to <
400>
18 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete(i) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
24 or a nucleotide sequence capable of hybridising to <
400>
24 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 33)
-
-
2. A method of improving plant productivity said method comprising introducing into said plant or propagation material thereof:
-
(i) an effective number of endophytic actinomycetes or variants, mutants or homologues thereof, which actinomycetes facilitate induction of at least one characteristic related to improved productivity; and
/or(ii) an effective amount of one or more metabolites derived from the actinomycetes of (i) or derivative, homologue, analogue, chemical equivalent or mimetic thereof;
for a time and under conditions sufficient to induce, in the subject plant, said characteristic, and wherein said actinomycete is selected from;
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
3 or a nucleotide sequence capable of hybridising to <
400>
3 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(b) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
4 or a nucleotide sequence capable of hybridising to <
400>
4 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
5 or a nucleotide sequence capable of hybridising to <
400>
5 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(d) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
6 or a nucleotide sequence capable of hybridising to <
400>
6 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
8 or a nucleotide sequence capable of hybridising to <
400>
8 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(f) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
9 or a nucleotide sequence capable of hybridising to <
400>
9 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
11 or a nucleotide sequence capable of hybridising to <
400>
11 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(h) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
14 or a nucleotide sequence capable of hybridising to <
400>
14 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(i) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
15 or a nucleotide sequence capable of hybridising to <
400>
15 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(j) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
17 or a nucleotide sequence capable of hybridising to <
400>
17 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete,(k) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
19 or a nucleotide sequence capable of hybridising to <
400>
19 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(l) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
20 or a nucleotide sequence capable of hybridising to <
400>
20 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(m) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
21 or a nucleotide sequence capable of hybridising to <
400>
21 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(n) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
22 or a nucleotide sequence capable of hybridising to <
400>
22 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(o) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
23 or a nucleotide sequence capable of hybridising to <
400>
23 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(p) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
25 or a nucleotide sequence capable of hybridising to <
400>
25 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(q) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
26 or a nucleotide sequence capable of hybridising to <
400>
26 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(r) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
27 or a nucleotide sequence capable of hybridising to <
400>
27 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(s) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
28 or a nucleotide sequence capable of hybridising to <
400>
28 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(t) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
29 or a nucleotide sequence capable of hybridising to <
400>
29 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete(u) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
30 or a nucleotide sequence capable of hybridising to <
400>
30 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.
-
-
31. A method of improving plant productivity said method comprising introducing into said plant or propagation material thereof:
-
(i) an effective number of at least teo endophytic actinomycete strains or variants, mutants or homologues thereof, which actinomycetes facilitate induction of at least one characteristic related to improved productivity; and
/or(ii) an effective amount of one or more metabolites derived from the actinomycetes of (i) or derivative, homologue, analogue, chemical equivalent or mimetic thereof;
for a time and under conditions sufficient to induce, in the subject plant, said characteristic, and wherein said at least two endophytic actinomycete strains are selected from;
(a) EN2, EN9 and EN23 (b) EN9, EN27 and EN28 (c) EN39 and EN46.
-
-
34. An agricultural composition comprising an endophytic actinomycete or metabolite derived therefrom together with one or more agriculturally acceptable carriers and/or diluents wherein said actinomycete is selected from:
-
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
1 or a nucleotide sequence capable of hybridising to <
400>
1 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(b) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
2 or a nucleotide sequence capable of hybridising to <
400>
2 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
7 or a nucleotide sequence capable of hybridising to <
400>
7 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(d) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
10 or a nucleotide sequence capable of hybridising to <
400>
10 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
12 or a nucleotide sequence capable of hybridising to <
400>
12 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(f) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
13 or a nucleotide sequence capable of hybridising to <
400>
13 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
16 or a nucleotide sequence capable of hybridising to <
400>
16 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(h) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
18 or a nucleotide sequence capable of hybridising to <
400>
18 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete(i) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
24 or a nucleotide sequence capable of hybridising to <
400>
24 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.
-
-
35. An agricultural composition comprising an endophytic actinomycete or metabolite derived therefrom together with one or more agriculturally acceptable carriers and/or diluents wherein said actinomycete is selected from:
-
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
3 or a nucleotide sequence capable of hybridising to <
400>
3 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(b) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
4 or a nucleotide sequence capable of hybridising to <
400>
4 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
5 or a nucleotide sequence capable of hybridising to <
400>
5 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(d) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
6 or a nucleotide sequence capable of hybridising to <
400>
6 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
8 or a nucleotide sequence capable of hybridising to <
400>
8 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(f) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
9 or a nucleotide sequence capable of hybridising to <
400>
9 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
11 or a nucleotide sequence capable of hybridising to <
400>
11 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(h) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
14 or a nucleotide sequence capable of hybridising to <
400>
14 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(i) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
15 or a nucleotide sequence capable of hybridising to <
400>
15 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(j) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
17 or a nucleotide sequence capable of hybridising to <
400>
17 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(k) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
19 or a nucleotide sequence capable of hybridising to <
400>
19 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(l) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
20 or a nucleotide sequence capable of hybridising to <
400>
20 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(m) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
21 or a nucleotide sequence capable of hybridising to <
400>
21 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(n) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
22 or a nucleotide sequence capable of hybridising to <
400>
22 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(o) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
23 or a nucleotide sequence capable of hybridising to <
400>
23 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(p) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
25 or a nucleotide sequence capable of hybridising to <
400>
25 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(q) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
26 or a nucleotide sequence capable of hybridising to <
400>
26 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(r) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
27 or a nucleotide sequence capable of hybridising to <
400>
27 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(s) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
28 or a nucleotide sequence capable of hybridising to <
400>
28 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(t) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
29 or a nucleotide sequence capable of hybridising to <
400>
29 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete(u) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
30 or a nucleotide sequence capable of hybridising to <
400>
30 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete. - View Dependent Claims (43, 44)
-
-
36. A novel, isolated endophytic actinomycete or variant, mutant or homologue thereof wherein said actinomycete is selected from:
-
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
1 or a nucleotide sequence capable of hybridising to <
400>
1 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(b) The actinomycete of (a) wherein said actinomycete corresponds to EN2 (AGAL Deposit No. NMO3/35895). (c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
2 or a nucleotide sequence capable of hybridising to <
400>
2 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(d) The actinomycete of (c) wherein said actinomycete corresponds to EN3 (AGAL Deposit No. NM03/36501). (e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
7 or a nucleotide sequence capable of hybridising to <
400>
7 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(f) The actinomycete of (e) wherein said aci corresponds to EN16 (AGAL Deposit No. NM03/35605). (g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
10 or a nucleotide sequence capable of hybridising to <
400>
10 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(h) The actinomycete of (g) wherein said actinomycete corresponds to EN23 (AGAL Deposit No. NM03/35605). (i) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
12 or a nucleotide sequence capable of hybridising to <
400>
12 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(j) The actinomycete of (i) wherein said actinomycete corresponds to EN27 (AGAL Deposit No. NM03/35606). (k) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
13 or a nucleotide sequence capable of hybridising to <
400>
13 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(l) The actinomycete of (i) wherein said actinomycete corresponds to EN28 (AGAL Deposit No. NM03/35607). (m) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
16 or a nucleotide sequence capable of hybridising to <
400>
16 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(n) The actinomycete of (m) wherein said actinomycete corresponds to EN46 (AGAL Deposit No. NM03/34609). (o) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
18 or a nucleotide sequence capable of hybridising to <
400>
18 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(p) The actinomycete of (o) wherein said actinomycete corresponds to EN60 (AGAL Deposit No. NM03/35896). (q) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
24 or a nucleotide sequence capable of hybridising to <
400>
24 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(r) The actinomycete of (q) wherein said actinomycete corresponds to PM87 (AGAL Deposit No. NM03/35608). - View Dependent Claims (38, 39, 40, 41, 42, 45)
-
-
37. A novel, isolated endophytic actinomycete or variant, mutant or homologue thereof wherein said actinomycete is selected from:
-
(a) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
3 or a nucleotide sequence capable of hybridising to <
400>
3 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(b) The actinomycete according to (a) wherein said actinomycete corresponds to EN5. (c) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
4 or a nucleotide sequence capable of hybridising to <
400>
4 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(d) The actinomycete of (c) wherein said actinomycete corresponds to EN6. (e) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
5 or a nucleotide sequence capable of hybridising to <
400>
5 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(f) The actinomycete of (e) wherein said actinomycete corresponds to EN7. (g) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
6 or a nucleotide sequence capable of hybridising to <
400>
6 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(1) The actinomycete of (g) wherein said actinomycete corresponds to EN9. (i) The actinomycete of (h) wherein said actinomycete is characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
8 or a nucleotide sequence capable of hybridising to <
400>
8 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(t) The actinomycete of (i) wherein said actinomycete corresponds to EN17. (k) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
9 or a nucleotide sequence capable of hybridising to <
400>
9 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(l) The actinomycete of (k) wherein said actinomycete corresponds to EN19. (m) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
11 or a nucleotide sequence capable of hybridising to <
400>
11 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(n) The actinomycete of (m) wherein said actinomycete corresponds to EN26. (o) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
14 or a nucleotide sequence capable of hybridising to <
400>
14 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(p) The actinomycete of (o) wherein said subject actinomycete corresponds to EN35. (q) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
15 or a nucleotide sequence capable of hybridising to <
400>
15 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(r) The actinomycete of (q) wherein said actinomycete corresponds to EN39. (s) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
17 or a nucleotide sequence capable of hybridising to <
400>
17 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(t) The actinomycete of (s) wherein said actinomycete corresponds to EN57. (u) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
19 or a nucleotide sequence capable of hybridising to <
400>
19 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(v) The actinomycete of (u) wherein said actinomycete corresponds to SE1. (w) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
20 or a nucleotide sequence capable of hybridising to <
400>
20 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(x) The actinomycete of (w) wherein said actinomycete corresponds to SE2. (y) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
21 or a nucleotide sequence capable of hybridising to <
400>
21 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(z) The actinomycete of (y) wherein said actinomycete corresponds to PM36. (aa) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
22 or a nucleotide sequence capable of hybridising to <
400>
22 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(ab) The actinomycete of (aa) wherein said actinomycete corresponds to PM40. (ac) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
23 or a nucleotide sequence capable of hybridising to <
400>
23 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(ad) The actinomycete of (ac) wherein said actinomycete corresponds to PM41. (ae) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
25 or a nucleotide sequence capable of hybridising to <
400>
25 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(af) The actinomycete of (ae) wherein said actinomycete corresponds to PM171. (ag) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
26 or a nucleotide sequence capable of hybridising to <
400>
26 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(ah) The actinomycete of (ag) wherein said actinomycete corresponds to PM185. (ai) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
27 or a nucleotide sequence capable of hybridising to <
400>
27 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(aj) The actinomycete of (ai) wherein said actinomycete corresponds to PM208. (ak) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
28 or a nucleotide sequence capable of hybridising to <
400>
28 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(al) The actinomycete of (ak) wherein said actinomycete corresponds to PM228. (am) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
29 or a nucleotide sequence capable of hybridising to <
400>
29 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(an) The actinomycete of (am) wherein said actinomycete corresponds to PM252. (ao) An actinomycete characterised either by a nucleotide sequence corresponding to the nucleotide sequence substantially as set forth in <
400>
30 or a nucleotide sequence capable of hybridising to <
400>
30 under low stringency conditions at 42°
C. or a variant, mutant or homologue of said actinomycete.(ap) The actinomycete of (ao) wherein said actinomycete corresponds to PM342.
-
Specification