Process for the preparation of high purity phytosterols from deodourizer distillate from vegetable oils
First Claim
1. A process for the preparation of high purity phytosterols from deodourizer distillate from vegetable oils which comprises mixing deodourizer distillate from vegetable oils with a methanolic solution of sulfuric acid in a ratio of 1:
- 1 to 1;
1.5 (v/v), stirring the above said reaction mixture, at a temperature of 50-85°
C., for a period of 2-4 hrs, distilling of the excess of methanol from the above said reaction mixture under vacuum, adding brine solution to the above said reaction mixture to separate sulfuric acid and removing the lower layer to obtain the ester layer, washing the above said ester layer with hot water till all of the acid and salts are removed, followed by crystallization at a temperature in the range of 0-25°
C. for a period of 110 hrs, filtering and washing the resultant crystals obtained with chilled n-hexane, at a temperature of 0-10°
C. to obtain the desired high purity phytosterols.
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Abstract
The present invention provides an isolation process of the pure sterols from the SODD by simple acid catalyzed esterification and the separation of the reagents by distillation and water washing followed by the crystallization of phytosterols and purification. Soybean oil deodorizer distillate (SODD) and distillate from other vegetable oil refining contain free sterols, in addition to steryl esters, tocopherols squalene and unknown compounds. The process is also applicable to other vegetable oil distillate containing more than 1% phytosterols in the free form. Phytosterols thus obtained is of high purity (95-99%) and high yield (80-90%) and contains β-sitosterol, Stigmasterol and campesterol.
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Citations
8 Claims
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1. A process for the preparation of high purity phytosterols from deodourizer distillate from vegetable oils which comprises mixing deodourizer distillate from vegetable oils with a methanolic solution of sulfuric acid in a ratio of 1:
- 1 to 1;
1.5 (v/v), stirring the above said reaction mixture, at a temperature of 50-85°
C., for a period of 2-4 hrs, distilling of the excess of methanol from the above said reaction mixture under vacuum, adding brine solution to the above said reaction mixture to separate sulfuric acid and removing the lower layer to obtain the ester layer, washing the above said ester layer with hot water till all of the acid and salts are removed, followed by crystallization at a temperature in the range of 0-25°
C. for a period of 110 hrs, filtering and washing the resultant crystals obtained with chilled n-hexane, at a temperature of 0-10°
C. to obtain the desired high purity phytosterols. - View Dependent Claims (2, 3, 4, 5, 6, 7)
- 1 to 1;
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8. A process for the preparation of high purity phytosterols from deodourizer distillate from vegetable oils, substantially as herein described with reference to the examples accompanying this specification.
Specification