Nuclease Inhibitor Cocktail
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Abstract
Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.
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Citations
70 Claims
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1-50. -50. (canceled)
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51. A method comprising:
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a) obtaining at least a first soluble anti-nuclease antibody;
b) obtaining at least a second soluble anti-nuclease antibody wherein said first and said at least second soluble anti-nuclease antibodies bind to different nucleases;
c) obtaining a composition; and
d) admixing the anti-nuclease antibodies and the composition to form an admixture;
wherein nucleases that may be present in the admixture are inhibited and wherein at least one of the first and second soluble anti-nuclease antibodies is a monoclonal antibody. - View Dependent Claims (52, 53, 54, 55, 56, 57, 58, 59)
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60. A method comprising:
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a) obtaining at least a first nuclease inhibitor further defined as a soluble anti-nuclease monoclonal antibody;
b) obtaining at least a second nuclease inhibitor further defined as a non-antibody nuclease inhibitor;
c) obtaining a composition; and
d) admixing the first nuclease inhibitor, the second nuclease inhibitor, and the composition to form an admixture;
wherein nucleases that may be present in the admixture are inhibited. - View Dependent Claims (61, 62, 63, 64, 65)
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66. A kit comprising:
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a) a first soluble anti-ribonuclease monoclonal antibody that binds to at least a first ribonuclease;
b) at least one of;
a second soluble anti-nuclease antibody that binds to a nuclease that is different from the first nuclease, or a non-antibody ribonuclease inhibitor; and
c) a reagent used in molecular biology. - View Dependent Claims (67, 68, 69, 70)
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Specification