Dual Path Immunoassay Device
First Claim
1. A test device for use with a solution and for use with a conjugate having a marker for determining the presence of a ligand in a liquid sample, the test device comprising:
- a) a first sorbent strip having a first location for receiving a solution and defining a first migration path for the solution and the conjugate;
b) a second sorbent strip distinct from said first sorbent strip having a second location for receiving the liquid sample and defining a second migration path for the sample distinct from said first migration path; and
c) a test site located on or in said first sorbent strip and/or said second sorbent strip, said test site having an immobilized ligand-binding mechanism, and said first and second sorbent strips touching each other at the test site, and said second migration path extending laterally from said second location to at least said test site such that application of the liquid sample to the second location requires time for the liquid sample to laterally flow to the test site and does not immediately wet the test site upon application.
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Accused Products
Abstract
The systems of the invention include test cells with a first sorbent material defining a first flow path for a solution, a second sorbent material defining a second flow path distinct from the first flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules such as aptamers, nucleic acids, etc. located at the junction of the first and second sorbent materials. The first and second sorbent strips touch each other at the test site location.
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Citations
30 Claims
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1. A test device for use with a solution and for use with a conjugate having a marker for determining the presence of a ligand in a liquid sample, the test device comprising:
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a) a first sorbent strip having a first location for receiving a solution and defining a first migration path for the solution and the conjugate;
b) a second sorbent strip distinct from said first sorbent strip having a second location for receiving the liquid sample and defining a second migration path for the sample distinct from said first migration path; and
c) a test site located on or in said first sorbent strip and/or said second sorbent strip, said test site having an immobilized ligand-binding mechanism, and said first and second sorbent strips touching each other at the test site, and said second migration path extending laterally from said second location to at least said test site such that application of the liquid sample to the second location requires time for the liquid sample to laterally flow to the test site and does not immediately wet the test site upon application. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method for testing a sample for the presence of a ligand, comprising:
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a) obtaining a test device having a first sorbent strip having a first location for receiving a solution and defining a first migration path for the solution and for a conjugate having a marker, a second sorbent material having a second location for receiving the liquid sample and defining a second migration path for the sample distinct from said first migration path, and a test site located on or in said first sorbent strip and/or said second sorbent strip, said test site having an immobilized ligand-binding mechanism, and said first and second sorbent strips touching each other at the test site location and said second migration path extending laterally from said second location to at least said test site such that application of the liquid sample to the second location requires time for the liquid sample to laterally flow to the test site and does not immediately wet the test site upon application;
b) applying the sample to said second location;
c) after said applying the sample, applying the solution to the first location;
and d) inspecting said test site to determine an indication the presence or lack thereof of the ligand in the sample. - View Dependent Claims (25, 26)
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27. A test device for determining the presence of a ligand in a liquid sample, comprising:
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a) a first sorbent strip having a first location for receiving a solution and defining a first migration path;
b) a second sorbent material having a second location for receiving the liquid sample and defining a second migration path distinct from said first migration path, said second migration path including a conjugate of an antibody bound to an interim binding agent, said antibody being selected to bind to the ligand when present in the sample;
c) a test site located on or in one at least one of said first sorbent strip and said second sorbent strip, said test site having an immobilized binding agent, said interim binding agent and said immobilized binding agent chosen for their ability to selectively bind well to each other, said first and second sorbent strips touching each other at the test site location. - View Dependent Claims (28, 29, 30)
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Specification