COMPOSITE MICROARRAY SLIDES

• US 20070148783A1
• Filed: 10/13/2006
• Published: 06/28/2007
• Est. Priority Date: 07/05/2000
• Status: Abandoned Application

• Current Assignee: 3M Innovative Properties Company (3M Company)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: MEYERING, Mark, SPEARIN, Aaron, DAIGLE, Derek, SOLOMON, Keith
• Application Number:
  US11/549,233
• Publication Number:
  US 20070148783A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00533 essentially rectangular
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B32B 17/10018 comprising only one glass sheet
• CPC Class Codes: B32B 17/10651 comprising colorants, e.g. dyes or pigments
• CPC Class Codes: B32B 17/10724 containing polyamide
• CPC Class Codes: B32B 2305/026 Porous
• CPC Class Codes: B32B 27/08 of synthetic resin
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G01N 33/551 : the carrier being inorganic

Abstract

Improved composite microarray slides for use in micro-analytical diagnostic applications are disclosed. Specifically, composite microarray slides useful for carrying a microarray of biological polymers on the surface thereof including composite microarray slides having a porous membrane formed by a phase inversion process effectively attached by covalent bonding through chemical agents that comprise anchor/linker moieties to a substrate that prepares the substrate to sufficiently bond to the porous membrane formed by a phase inversion process such that the combination produced thereby is useful in microarray applications and wherein the composite microarray slides are covalently bonded to a solid base member, such as, for example, a glass or Mylar microscope slide, such that the combination produced thereby is useful in microarray applications. Apparatus and methods for fabricating the composite microarray slides are also disclosed.

Claims

1. A method of fabricating composite microarray slides useful for carrying a microarray of biological polymers comprising the acts of:

providing a non-porous substrate;

providing a microporous membrane formed by a phase inversion process;

providing a surface treatment, wherein the surface treatment comprises organosilanes;

applying the surface treatment to the non-porous substrate; and

operatively associating the non-porous substrate having the surface treatment applied thereto with the microporous membrane for forming an attachment layer therebetween that when subjected to an organic solvent system for greater than about six hours, the microporous membrane does not delaminate significantly from the non-porous substrate, the non-porous substrate is sufficiently bonded to the microporous membrane to withstand challenging environments encountered in microarray applications.

2. The method of claim 1 wherein the attachment layer covalently bonds the non-porous substrate and the microporous membrane.

3. The method of claim 1 wherein the microporous membrane substantially covers the surface of the non-porous substrate.

4. The method of claim 1 wherein applying the surface treatment produces an attachment layer having minimal thickness.

5. The surface treatment of claim 4, wherein the attachment layer is between about 0.1 to about 12 microns thick.

6. The surface treatment of claim 4, wherein the attachment layer is between about 2 to about 5 microns thick.

7. The surface treatment of claim 4, wherein the attachment layer is about 3 microns thick.

8. The method of claim 1, wherein the operatively associated of the non-porous substrate to the microporous membrane forms a uniform attachment layer.

9. The method of claim 1 wherein the attachment layer is applied to the non-porous substrate producing a minimal finite thickness or mass which adds uniformity to the overall thickness of the composite microarray slide.

10. The method of claim 1 wherein applying the attachment layer at least substantially eliminates nonuniformity of the overall thickness of the substrate/membrane combination structure.

11. The method of claim 1 further comprising:

providing a microporous membrane containing a sufficient amount of pigments.

12. The microporous membrane of claim 11 wherein the pigments comprise:

carbon-black.

13. The method of claim 1 wherein the microporous membrane, prior to being operatively associated with the non-porous substrate, is wet-as-cast.

14. The method of claim 1, wherein the microporous membrane, prior to being operatively associated with the non-porous substrate, is substantially dry.

15. The microporous membrane of claim 1 wherein the microporous membrane is asymmetric.

16. The microporous membrane of claim 1 wherein the microporous membrane is symmetric.

17. The composite microarray slide of claim 1 wherein the presence of the attachment layer results in minimal interference in the binding of the biological polymer.

18. The method of claim 1 wherein the presence of the attachment layer results in minimal interference the detection of the biological polymers.

19. The method of claim 1 wherein when subjected to 4×

SSC at about 60°

C. for greater than about 10 hours, the microporous membrane does not delaminate significantly from the non-porous substrate.

20. The method of claim 1 wherein when subjected to 4×

SSC at about 60°

C. for about 2 weeks, the microporous membrane does not delaminate significantly from the non-porous substrate.