Method for detecting Nipah virus and method for providing immunoprotection against Henipa viruses

• US 20070150972A1
• Filed: 09/08/2006
• Published: 06/28/2007
• Est. Priority Date: 09/22/2003
• Status: Abandoned Application

• Current Assignee: Institut Pasteur
• Original Assignee: Institut Pasteur
• Inventors: Deubel, Vincent, Wild, Fabian, Loth, Philippe, Contamin, Hugues, George Courbot, Marie-Claude, Kum, Thong Wong, Buckland, Robin
• Application Number:
  US11/517,464
• Publication Number:
  US 20070150972A1
• Time in Patent Office: Days
• US Class Current: 800/14
• CPC Class Codes: A01K 2227/10 Mammal
• CPC Class Codes: A01K 2267/0337 Animal models for infectious diseases
• CPC Class Codes: A01K 67/027 New or modified breeds of vertebrates
• CPC Class Codes: A61K 2039/5256 expressing foreign proteins
• CPC Class Codes: A61K 38/162 from virus
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/155 Paramyxoviridae, e.g. parainfluenza virus
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C07K 16/1027 Paramyxoviridae, e.g. respiratory syncytial virus
• CPC Class Codes: C07K 2317/76 Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2710/24143 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 2760/18211 Henipavirus, e.g. hendra virus
• CPC Class Codes: C12N 2760/18222 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2760/18234 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: C12Q 2531/113 : PCR
• CPC Class Codes: C12Q 2545/114 : involving a quantitation step

Abstract

The present invention provides an animal model for monitoring Nipah virus infection, a method for the quantitative detection and rapid characterization of Nipah virus RNA in a sample, a composition which can be used to provide immunoprotection in an individual as well as monoclonal antibodies which neutralize Nipah and Hendra virus and can be used for prophylaxis, treatment, and/or prevention.

Claims

1. An golden hamster animal model of Henipavirus infection, which is infected with a Henipavirus.

2. A method of detecting Nipah virus in a sample, comprising:

producing a DNA copy of at least one RNA molecule of said Nipah virus with at least one primer specific for the RNA molecule;

amplifying the DNA copy with at least one pair of oligonucleotide primers specific for the DNA copy of the Nipah virus RNA molecule; and

detecting the presence of an amplified DNA corresponding to Nipah virus, which is indicative of the presence of Nipah virus in the sample.

3. The method of claim 2, wherein the DNA copy produced and amplified is a Nipah virus nucleocapsid coding region.

4. The method of claim 2, wherein at least one of the pair of oligonucleotide primers comprises a detectable moiety.

5. The method of claim 4, wherein the detecting comprises visualizing the detectable moiety.

6. The method of claim 2, wherein the sample is obtained from a pig.

7. The method of claim 2, wherein the sample is obtained from a wild or domestic animal

8. The method of claim 2, wherein the sample is obtained from a human.

9. The method of claim 2, wherein the at least one primer specific for the RNA molecule comprises at least 15 consecutive nucleotides of complementary to a polynucleotide which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:

16, SEQ ID NO;

23, SEQ ID NO;

31 and SEQ ID NO;

32.

10. The method of claim 9, wherein the at least one primer specific for the RNA molecule comprises at least 20 consecutive nucleotides of the polynucleotide.

11. The method of claim 9, wherein the at least one primer specific for the RNA molecule comprises at least 25 consecutive of the polynucleotide.

12. A method of protecting an individual against a Henipavirus infection comprising:

administering the at least one isolated Henipavirus G and F glycoproteins to said individual or mammal in an amount sufficient to induce an immune response in said individual or mammal.

13. The method of claim 12 wherein the Henipavirus is Nipah or Hendra virus.

14. The method of claim 12, wherein said administering further comprises administering an adjuvant.

15. The method of claim 12, wherein said administering is performed one or more times.

16. The method of claim 15, wherein at least both the Henipavirus F and G glycoproteins are administered.

17. A method of preventing or protecting an individual or mammal in need thereof against Henipavirus infection comprising:

administering an expression vector, which expresses at least one isolated Henipavirus G and F glycoproteins to said individual or mammal in an amount sufficient to induce an immune response in said individual or mammal to prevent or protect the individual or mammal against Henipavirus infection.

18. The method of claim 17, wherein the expression vector expresses at least F and G glycoproteins of the Henipavirus.

19. The method of claim 17, wherein the expression vector is a viral vector.

20. The method of claim 19, wherein the viral vector is a recombinant poxvirus vector.

21. The method of claim 17, further comprising administering at least one adjuvant.

22. A recombinant hybridoma which produces an antibody against one or both of a Henipavirus G or F protein.

23. A recombinant poxvirus vector expressing one or both of a Henipavirus G or F protein.

24. A recombinant vaccinia virus expressing Nipah G protein deposited at the CNCM as No. I-3086.

25. A recombinant vaccinia virus expressing Nipah F protein deposited at the CNCM as No. I-3085.

26. Hybridoma N°

1.7 anti-Nipah virus G protein with neutralizing activity against Nipa virus.

27. Hybridoma N°

3.B10 anti-Nipah virus G protein with neutralizing activity against Nipa virus.

28. Hybridoma N°

35 anti-Nipah virus F protein with neutralizing activity against Nipah and Hendra virus.

29. Hybridoma N°

3 anti-Nipah virus F protein with neutralizing activity against Nipah and Hendra virus.