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MOLECULAR DIAGNOSTICS AMPLIFICATION SYSTEM AND METHODS

  • US 20070154922A1
  • Filed: 12/15/2006
  • Published: 07/05/2007
  • Est. Priority Date: 12/29/2005
  • Status: Active Grant
First Claim
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1. A single-use nucleic acid amplification device for producing an amplicon, comprising:

  • a housing; and

    an amplification chamber, comprising;

    an ingress with a first reversible seal;

    an egress with a second reversible seal;

    a sealable sample entry orifice; and

    a first wall forming a portion of the amplification chamber,wherein the first wall comprises a thermally conductive material and includes a first surface and an second surface,wherein the second surface includes a heating circuit and a temperature sensor,wherein the sample entry orifice permits a sample of nucleic acid to enter the amplification chamber,wherein the ingress is connected to a first conduit along with a pump and a reservoir, andwherein the egress is connected to a second conduit permitting egress of the amplicon from the amplification chamber.

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