Method for Gridding and Quality Control of Polymer Arrays
First Claim
Patent Images
1. A method of monitoring hybridization of control sample sequences to control probe sequences on an array comprising:
- providing a high density polymer probe array having a plurality of test probes that bind to test sample targets and a plurality of control probes at predefined regions on the array and wherein the control probes bind to control sample targets;
the control probes further comprising a plurality of first control probes at predefined regions on the array and a plurality of second control probes at predefined regions on the array; and
wherein the first control probes are different from the second control probes;
contacting first control sample targets with the array; and
determining whether hybridization occurred between the first control sample targets and the first control probes.
3 Assignments
0 Petitions
Accused Products
Abstract
Methods of using a plurality of control probes for gridding of a nucleic acid array and for quality control purposes are disclosed.
23 Citations
38 Claims
-
1. A method of monitoring hybridization of control sample sequences to control probe sequences on an array comprising:
-
providing a high density polymer probe array having a plurality of test probes that bind to test sample targets and a plurality of control probes at predefined regions on the array and wherein the control probes bind to control sample targets;
the control probes further comprising a plurality of first control probes at predefined regions on the array and a plurality of second control probes at predefined regions on the array; and
wherein the first control probes are different from the second control probes;
contacting first control sample targets with the array; and
determining whether hybridization occurred between the first control sample targets and the first control probes. - View Dependent Claims (2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
-
7. The method of claim 7 wherein the bacterial genes are BioB, BioC, BioD or cre.
-
21. A method for quality control of an oligonucleotide array having probes thereon, wherein said array has a plurality of quality control blocks surrounded at the four corners of each with first checkerboard features, said method comprising,
providing a quality control block in the center of the array; -
placing at the four corners of the center control block, second checkerboard features, wherein control sample sequences that bind to the second checkerboard features do not bind to the first checkerboard features;
imaging the center control blocks and the second checkerboard features to allow for gridding of the array and a determination of whether the probes in the quality control blocks have been accurately synthesized. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
-
Specification