OLIGONUCLEOTIDES AND ANALOGS LABELED WITH ENERGY TRANSFER DYES
First Claim
1. A fluorescently-labeled reagent comprising an oligonucleotide or an oligonucleotide analog and an energy transfer dye, wherein energy transfer dye is covalently linked to the oligonucleotide or oligonucleotide analog, and comprises:
- a fluorescein donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto;
a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and
a non-nucleosidic linker linking the 5- or 6-position of the fluorescein donor dye to the 5- or 6-position of the 4,7-dichlororhodamine acceptor dye.
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Accused Products
Abstract
Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
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Citations
23 Claims
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1. A fluorescently-labeled reagent comprising an oligonucleotide or an oligonucleotide analog and an energy transfer dye, wherein energy transfer dye is covalently linked to the oligonucleotide or oligonucleotide analog, and comprises:
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a fluorescein donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto;
a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and
a non-nucleosidic linker linking the 5- or 6-position of the fluorescein donor dye to the 5- or 6-position of the 4,7-dichlororhodamine acceptor dye. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification