Cell division marker
First Claim
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1. A method of detecting the presence of a midbody scar in a mammalian cell, the method comprising:
- contacting a mammalian cell suspected to have a midbody scar with an antibody or antigen-binding portion thereof that binds specifically to a polypeptide component of a midbody scar selected from the group consisting of; and
detecting binding of the antibody to its antigen, wherein binding of the antibody indicates the presence of a midbody scar in the mammalian cell.
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Abstract
This application relates to a newly identified animal cell structure, the midbody scar. This structure is a remnant of the midbody that is retained by one daughter cell following cytokinesis and persists through multiple subsequent cell cycles. The midbody scar can be useful as a marker of dividing cells or of a cell'"'"'s replicative age.
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Citations
36 Claims
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1. A method of detecting the presence of a midbody scar in a mammalian cell, the method comprising:
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contacting a mammalian cell suspected to have a midbody scar with an antibody or antigen-binding portion thereof that binds specifically to a polypeptide component of a midbody scar selected from the group consisting of; and
detecting binding of the antibody to its antigen, wherein binding of the antibody indicates the presence of a midbody scar in the mammalian cell. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10)
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4. The method of claim 4, wherein the polypeptide is selected from the group consisting of mitotic kinesin-like protein 1 (MKLP1);
- Epsilon tubulin (ε
-tubulin);
Centrosomal Protein 55 kDa (Cep55); and
Aurora B kinase.
- Epsilon tubulin (ε
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11. A method of identifying a dividing cell in a population of mammalian cells, the method comprising:
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obtaining a population of mammalian cells comprising at least one cell suspected of being a dividing cell; and
detecting a cell having one or more midbody scars, wherein a cell having one or more midbody scars is identified as a dividing cell. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. An isolated midbody scar from a mammalian cell, wherein the midbody scar comprises mitotic kinesin-like protein 1 (MKLP1);
- Epsilon tubulin (ε
-tubulin);
Centrosomal Protein 55 kDa (Cep55); and
Aurora B kinase.
- Epsilon tubulin (ε
Specification