Chemiluminescence sensor array

• US 20070154967A1
• Filed: 12/30/2005
• Published: 07/05/2007
• Est. Priority Date: 12/30/2005
• Status: Active Grant

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Sundararajan, Narayan, Koo, Tae-Woong
• Granted Patent: US 7,537,934 B2
• Time in Patent Office: Days
• US Class Current: 435/7.920
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10T 29/49826 Assembling or joining

Abstract

Embodiments of the invention relate to integrated chemiluminescence devices and methods for monitoring molecular binding utilizing these devices and methods. These devices and methods can be used, for example, to identify antigen binding to antibodies. The devices include both a chemiluminescence material and a detector integrated together.

Claims

1. An integrated assay device comprising:

a chemiluminescent material;

a biopolymer; and

a light detector, wherein an activity of the biopolymer can activate the chemiluminescent material and the activity of the chemiluminescent material can be detected by the light detector.

2. The device of claim 1, wherein the biopolymer is an antibody.

3. The device of claim 1, further comprising a support substrate, wherein the chemiluminescent material, the biopolymer;

and the light detector are on the support substrate.

4. The device of claim 2, wherein the support substrate comprises glass, nickel, magnetic metal, gold, silicon, nitrocellulose, or polyvinylidene difluoride (PVDF).

5. The device of claim 2, wherein the antibody comprises a conjugate enzyme for activating the chemiluminescent material.

6. The device of claim 5, wherein the conjugate enzyme is horseradish peroxidase.

7. The device of claim 2, wherein the antibody is capable of activating the chemiluminescent material upon binding to an antigen.

8. The device of claim 2, wherein the antibody is configured to bind to an antigen of interest.

9. The device of claim 1, wherein the chemillminescent material comprises a cyclic diacylhydrazides.

10. The device of claim 1, wherein the chemiluminescent material comprises a material selected from the group consisting of luminol, N-(4-Aminobutyl)-N-ethylisoluminol, 4-Aminophthalhydrazide monohydrate, Bis(2-carbopentyloxy-3,5,6-trichlorophenyl) oxalate, 9,10-Bis(phenylethynyl)anthracene, 5,12-Bis(phenylethynyl)naphthacene, 2-Chloro-9,10-bis(phenylethynyl)anthracene, 1,8-Dichloro-9,10-bis(phenylethynyl)anthracene, Lucifer Yellow CH dipotassium salt, Lucifer yellow VS dilithium salt, 85% (dye content), 2,4,5-Triphenylimidazole, 9,10-Diphenylanthracene, Rubrene, and Tetrakis(dimethylamino)ethylene.

11. The device of claim 1, wherein the light detector is a charge-coupled device (CCD) or a complimentary metal-oxide semiconductor (CMOS).

12. A method of making an integrated device comprising:

applying a chemiluminescent material to a support substrate comprising a light detector; and

applying a biopolymer to the support.

13. The method of claim 12, wherein the biopolymer is an antibody.

14. The method of claim 12, wherein the support substrate comprises glass, nickel, magnetic metal, gold, silicon, nitrocellulose, or polyvinylidene difluoride (PVDF).

15. The method of claim 13, wherein the antibody comprises a conjugate enzyme for activating the chemiluminescent material.

16. The method of claim 15, wherein the conjugate enzyme is horseradish peroxidase.

17. The method of claim 13, wherein the antibody is capable of activating the chemiluminescent material upon binding to an antigen.

18. The method of claim 13, wherein the antibody is configured to bind to an antigen of interest.

19. The method of claim 12, wherein the chemiluminescent material comprises a cyclic diacylhydrazides.

20. The method of claim 12, wherein the chemiluminescent material comprises a material selected from the group consisting of luminol, N-(4-Aminobutyl)-N-ethylisoluminol, 4-Aminophthalhydrazide monohydrate, Bis(2-carbopentyloxy-3,5,6-trichlorophenyl) oxalate, 9,10-Bis(phenylethynyl)anthracene, 5,12-Bis(phenylethynyl)naphthacene, 2-Chloro-9,10-bis(phenylethynyl)anthracene, 1,8-Dichloro-9,10-bis(phenylethynyl)anthracene, Lucifer Yellow CH dipotassium salt, Lucifer yellow VS dilithium salt, 85% (dye content), 2,4,5-Triphenylimidazole, 9,10-Diphenylanthracene, Rubrene, and Tetrakis(dimethylamino)ethylene.

21. The method of claim 12, wherein the light detector is a charge-coupled device (CCD) or a complimentary metal-oxide semiconductor (CMOS).

22. A method of detecting an antigen comprising:

introducing an analyte antigen to a device comprising a chemiluminescent material, an antibody that binds to a target antigen and a light detector, wherein the chemiluminescent material luminesces if the analyte antigen is the target antigen.

23. The method of claim 22, wherein the device further comprises a support substrate and the chemiluminescent material, the antibody;

and the light detector are on the support substrate.

24. The method of claim 23, wherein the support substrate comprises glass, nickel, magnetic metal, gold, silicon, nitrocellulose, or polyvinylidene difluoride (PVDF).

25. The method of claim 22, wherein the antibody comprises a conjugate enzyme for activating the chemiluminescent material.

26. The method of claim 25, wherein the conjugate enzyme is horseradish peroxidase.

27. The method of claim 22, wherein the antibody is capable of activating the chemiluminescent material upon binding to an antigen.

28. The method of claim 22, wherein the chemiluminescent material comprises a cyclic diacylhydrazides.

29. The method of claim 22, wherein the chemiluminescent material comprises a material selected from the group consisting of luminol, N-(4-Aminobutyl)-N-ethylisoluuminol, 4-Aminophthalhydrazide monohydrate, Bis(2-carbopentyloxy-3,5,6-trichlorophenyl) oxalate, 9,10-Bis(phenylethynyl)anthracene, 5,12-Bis(phenylethynyl)naphthacene, 2-Chloro-9,10-bis(phenylethlynyl)anthracene, 1,8-Dichloro-9,10-bis(phenylethynyl)anthracene, Lucifer Yellow CH dipotassium salt, Lucifer yellow VS dilithium salt, 85% (dye content), 2,4,5-Triphenylimidazole, 9,10-Diphenylanthracene, Rubrene, and Tetrakis(dimethylamino)ethylene.

30. The method of claim 22, wherein the light detector is a charge-coupled device (CCD) or a complimentary metal-oxide semiconductor (CMOS).

31. The method of claim 22, wherein a metal-nanoparticle tethered antibody also capable of specifically binding to the target antigen is applied after the target antigen is bound to the antibody in the device.

32. A method of making an integrated device comprising:

attaching a light detector to a support comprising a chemiluminescent material and a biopolymer.

33. The method of claim 32, wherein the biopolymer is an antigen.

34. The method of claim 32, wherein the support substrate comprises glass, nickel, magnetic metal, gold, silicon, nitrocellulose, or polyvinylidene difluoride (PVDF).

35. The method of claim 33, wherein the antibody comprises a conjugate enzyme for activating the chemiluminescent material.

36. The method of claim 35, wherein the conjugate enzyme is horseradish peroxidase.

37. The method of claim 33, wherein the antibody is capable of activating the chemiluminescent material upon binding to an antigen.

38. The method of claim 33, wherein the antibody is configured to bind to an antigen of interest.

39. The method of claim 32, wherein the chemiluminescent material comprises a cyclic diacylhyrazides.

40. The method of claim 32, wherein the chemiluminescent material comprises a material selected from the group consisting of luminol, N-(4-Aminobutl)-N-ethylisoluminol, 4-Aminophtalhydrazide monohydrate, Bis(2-carbopentyloxy-3,5,6-trichlorophenyl)oxalate, 9,29-Bis(phenylethynyl)anthracene, 5,12-Bis(phenylethynyl)naphthacene, 2-Chloro-9,29-bis(phenylethynyl)anthracene, 1,8-Dichloro-9,29-bis(phenylethynyl)anthracenene, Lucifer Yellow CH dipotassium salt, Lucifer yellow VS dilithium salt, 85% (dye content), 2,4,5-Triphenylimidazole, 9,29-Diphenylanthracene, Rubrene, and Tetrakis(dimethylamino)ethylene.

41. The method of claim 32, wherein the light detector is a charge-coupled device (CCD) or a complimentary metal-oxide semiconductor (CMOS).