ETHANOL PRODUCTIVITIES OF SACCHAROMYCES CEREVISIAE STRAINS IN FERMENTATION OF DILUTE-ACID HYDROLYZATES DEPEND ON THEIR FURAN REDUCTION CAPACITIES

• US 20070155000A1
• Filed: 11/16/2006
• Published: 07/05/2007
• Est. Priority Date: 05/19/2004
• Status: Abandoned Application

• Current Assignee: Scandinavian Technology Group AB
• Original Assignee: Scandinavian Technology Group AB
• Inventors: Hahn-Hägerdal, Bärbel, Lidén, Gunnar, Gorwa-Grauslund, Marie-Francoise, Modig, Carl, Moreira De Almeida, Joao, Nilsson, Anneli
• Application Number:
  US11/560,490
• Publication Number:
  US 20070155000A1
• Time in Patent Office: Days
• US Class Current: 435/161
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12N 9/92 Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
• CPC Class Codes: C12P 17/04 containing a five-membered hetero ring, e.g. griseofulvin , vitamin C
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates to an ethanol producing microbial strain, such as Saccharomyces cerevisiae strain, being able to grow and produce ethanol from lignocellulosic hydrolysates comprising growth inhibiting compounds of the group furfural and 5-hydroxy-methyl furfural, in a batch, fed-batch or continuous fermentation, said microbial strain being tolerant to such inhibiting compounds, which strain is upregulated and/or over expressed with regard to one or more of the following genes: LAT1, ALD6, ADH5, ADH6, GDH3, OYE3, SER3, GND2, MDH2, IDP3, ADH7, AAD15, ERG27, HMG1, LYS5, SPS19, SGE1.

Claims

1. Ethanol producing Saccharomyces cerevisiae strain, wherein;

a) said strain is able to grow and produce ethanol from lignocellulosic hydrolysates comprising growth inhibiting compounds of the group furfural and 5-hydroxy-methyl furfural, in a batch, fed-batch or continuous fermentation and;

b) said strain being tolerant to such inhibiting compounds; and

c) said strain is upregulated and/or overexpressed with regard to one or more of the following genes;

LAT1, ALD6, ADH5, ADH6, GDH3, OYE3, SER3, GND2, MDH2, IDP3, ADH7, AAD15, ERG27, HMG1, LYS5, SPS19, SGE1; and

d) said strain overexpresses xylose reductase, xylitol dehydrogenase or xylose isomerase genes and is upregulated with regard to xylulose kinase.

2. Ethanol producing strain according to claim 1, wherein the strain is upregulated and/or overexpressed with regard to one or more of the genes SFA1, ADH2, ADH6, ADH7, SPS19.

3. Ethanol producing I strain according to claim 1, wherein the upregulated and/or overexpressed gene is an alcohol dehydrogenase (ADH).

4. Ethanol producing I strain according to claim 1, wherein the upregulated and/or overexpressed gene is ADH6.

5. Ethanol producing strain according to claim 1, wherein the alcohol dehydrogenase is NADPH dependent.

6. The application of the strain according to claim 1, for the reduction of HMF in a lignocellulosic medium when fermenting said medium to produce ethanol.

7-10. -10. (canceled)