FRAGMENTING IONS IN MASS SPECTROMETRY
First Claim
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1. ) A method of controlling the fragmentation of ions during mass spectral analysis, comprising:
- (i) at a starting collision energy provided within a mass spectrometer, fragmenting at least one of a plurality of precursor ions generated from a sample to produce a plurality of daughter ion fragments;
(ii) determining an ion current associated with unfragmented precursor ions in the mass spectrometer;
(iii) determining an ion current associated with the daughter ion fragments in the mass spectrometer;
(iv) determining the ratio of the current associated with the unfragmented precursor ions to the current associated with the daughter ion fragments; and
(v) adjusting the collision energy provided in the mass spectrometer at (i) to move the ratio toward a predetermined range or value.
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Abstract
Systems, methods, and computer program products useful in controlling the fragmentation of ions. Control of fragmentation is achieved by varying the collision energy imparted to precursor ions.
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Citations
12 Claims
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1. ) A method of controlling the fragmentation of ions during mass spectral analysis, comprising:
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(i) at a starting collision energy provided within a mass spectrometer, fragmenting at least one of a plurality of precursor ions generated from a sample to produce a plurality of daughter ion fragments;
(ii) determining an ion current associated with unfragmented precursor ions in the mass spectrometer;
(iii) determining an ion current associated with the daughter ion fragments in the mass spectrometer;
(iv) determining the ratio of the current associated with the unfragmented precursor ions to the current associated with the daughter ion fragments; and
(v) adjusting the collision energy provided in the mass spectrometer at (i) to move the ratio toward a predetermined range or value. - View Dependent Claims (2, 3, 4)
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5. ) A system useful for controlling the fragmentation of ions during mass spectral analysis, the system comprising a controller adapted to:
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(i) at a starting collision energy provided within a mass spectrometer, fragment at least one of a plurality of precursor ions generated from a sample to produce a plurality of daughter ion fragments;
(ii) determine an ion current associated with unfragmented precursor ions in the mass spectrometer;
(iii) determine an ion current associated with the daughter ion fragments in the mass spectrometer;
(iv) determine the ratio of the current associated with the unfragmented precursor ions to the current associated with the daughter ion fragments; and
(v) adjust the collision energy provided in the mass spectrometer at (i) to move the ratio toward a predetermined range or value. - View Dependent Claims (6, 7, 8)
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9. ) A computer usable medium having computer readable code embodied therein for causing a mass spectrometer to:
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(i) at a starting collision energy provided within a mass spectrometer, fragment at least one of a plurality of precursor ions generated from a sample to produce a plurality of daughter ion fragments;
(ii) determine an ion current associated with unfragmented precursor ions in the mass spectrometer;
(iii) determine an ion current associated with the daughter ion fragments in the mass spectrometer;
(iv) determine the ratio of the current associated with the unfragmented precursor ions to the current associated with the daughter ion fragments; and
(v) adjust the collision energy provided in the mass spectrometer at (i) to move the ratio toward a predetermined range or value. - View Dependent Claims (10, 11, 12)
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Specification