Microfluidic systems for size based removal of red blood cells and platelets from blood
First Claim
1. A device for concentrating particles, the device comprising:
- a. a channel having an inlet and first and second outlets;
b. a first sieve disposed between the inlet and the first outlet, wherein the first sieve is not disposed between the inlet and the second outlet; and
c. a force generator to direct particles to the first sieve.
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Accused Products
Abstract
The invention features devices and methods for enriching a sample in one or more desired particles. An exemplary use of these devices and methods is for the enrichment of cells, e.g., white blood cells in a blood sample. In general, the methods of the invention employ a device that contains at least one sieve through which particles of a given size, shape, or deformability can pass. Devices of the invention have at least two outlets, and the sieve is placed such that a continuous flow of fluid can pass through the device without passing through the sieve. The devices also include a force generator for directing selected particles through the sieve. Such force generators employ, for example, diffusion, electrophoresis, dielectrophoresis, centrifugal force, or pressure-driven flow.
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Citations
63 Claims
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1. A device for concentrating particles, the device comprising:
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a. a channel having an inlet and first and second outlets;
b. a first sieve disposed between the inlet and the first outlet, wherein the first sieve is not disposed between the inlet and the second outlet; and
c. a force generator to direct particles to the first sieve. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of producing, from a particle-containing fluid, a sample enriched in a target population of particles, the method comprising the steps of:
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a. providing a device comprising;
i. a channel having an inlet and a first and a second outlet; and
ii. a first sieve disposed between the inlet and the first outlet, wherein the first sieve is not disposed between the inlet and the second outlet; and
iii. a force generator to direct particles to the first sieve;
b. directing the particle-containing fluid through the inlet into the channel;
c. actuating the force generator so that particles in the fluid are directed to the first sieve and do or do not substantially pass through the first sieve based on the size, shape, or deformability of the particles; and
d. collecting the effluent containing particles of the target population from the first outlet if the particles of the target population substantially pass through the first sieve or from the second outlet if the particles of the target population do not substantially pass through the first sieve, thereby producing the sample enriched in the target population of particles. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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- 22. A device for enriching a first cell type from a blood sample comprising a first inlet in communication with a channel wherein said channel comprises two rows of obstacles that direct said first cell type in a first direction and a second cell type in a second direction, and wherein said device comprises a first outlet in said first direction and a second outlet in said second direction.
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39. A device for enriching a first cell type from a fluid sample comprising said first cell type and a second cell type, said device comprising:
a first inlet fluidly coupled to a channel comprising a plurality of obstacles that direct said first cell type in a first direction and said second cell type in a second direction, wherein said first cell type is a cancer cell or a fetal red blood cell and wherein said device further comprises a first outlet in said first direction and a second outlet in said second direction. - View Dependent Claims (40, 41, 42, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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53. A method for enriching one or more fetal red blood cells in a fluid sample comprising fetal red blood cells and non-fetal red blood cells, said method comprising:
- applying said fluid sample to a device comprising a first inlet coupled to a channel comprising a plurality of obstacles that directs said one or more fetal red blood cells in a first direction and said one or more non-fetal red blood cells in a second direction, wherein said device further comprises a first outlet in said first direction and a second outlet in said second direction.
- View Dependent Claims (54, 55, 56)
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57. A method for enriching one or more cancer cells from a fluid sample comprising cancer cells and non-cancer cells, said method comprising:
- applying said fluid sample to a device comprising a first inlet coupled to a channel comprising a plurality of obstacles that directs said one or more cancer cells in a first direction and one or more non-cancer cells in a second direction, wherein said device further comprises a first outlet in said first direction and a second outlet in said second direction.
- View Dependent Claims (58, 59, 60)
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61. A method for enriching a first cell type from a blood sample comprising said first cell type and a second cell type, said method comprising:
- applying said blood sample to a device comprising a first inlet adapted for delivering said blood sample to a channel wherein said channel comprises two rows of obstacles that direct said first cell type in a first direction and a second cell type in a second direction, and wherein said device comprises a first outlet in said first direction and a second outlet in said second direction.
- View Dependent Claims (62, 63)
Specification