Device for analyzing an interaction between target and probe molecules

• US 20070160998A1
• Filed: 02/08/2005
• Published: 07/12/2007
• Est. Priority Date: 02/11/2004
• Status: Abandoned Application

• Current Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Original Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Inventors: Van Beuningen, Marinus
• Application Number:
  US10/586,882
• Publication Number:
  US 20070160998A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00292 in the shape of pipette tips
• CPC Class Codes: B01J 2219/00639 the compounds being trapped in or bound to a porous medium
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/1055 for immobilising reagents, e.g. dried reagents

Abstract

The present invention relates to elongated hollow tubular body for analysing an interaction between target and probe molecules, comprising a support with through going channels enabling flow through of liquids arranged at the open end, and a mouthpiece at the other end. In particular a flow-through array technology linked to a disposable in the form of a 0.1-1 ml standard pipet tip is provided. The device pre-spotted with a number of markers can be used in robotic/liquid handling system capable of handling disposable tips. The roboticaiquid handling system has the ability to perform all liquid handling steps through the syringes on the system including the binding of markers in the flow-through arrays. Furthermore all incubation steps can be performed on the platform which is equipped with an incubator device and an optical read-out device for analysing its signal.

Claims

1. A device for analyzing analysing an interaction between target and probe molecules, comprising:

a tubular housing having a proximal end and a distal end defining an internal flow passageway, and

a flow through support member provided within or on the housing obstructing said internal passageway, whereby said flow through support member is provided with through going channels suitable for allowing an interaction between target and probe molecules.

2. The device according to claim 1, wherein said support member is provided with probe molecules suitable for interacting with target molecules.

3. The device according to claim 1, whereby the support member is provided at or near the distal end of the housing.

4. The device according to claim 1, wherein said support member is chosen from the group consisting of metals, ceramic metal oxides, silicon, organic polymers and metal oxides, preferably aluminium oxide.

5. The device according to claim 1, wherein said support member is optically transparent or translucent.

6. The device according to claim 1, wherein said channels extend substantially coaxial with the longitudinal axis of the housing.

7. The device according to claim 1, wherein the plane of the support member extends substantially perpendicular to the longitudinal axis of the housing.

8. The device according to claim 1, wherein the support member spans the bore of the housing.

9. An apparatus for analyzing an interaction between target and probe molecules, comprising:

(a) a handling station comprising a handling device, for aspirating and/or dispensing fluid medium, said handling device comprising a device according to claim 1, mounted thereto,

(b) a means for transporting said handling station to a plurality of sections,

(c) at least one incubation section comprising an incubation device, for administering a fluid sample comprising target molecules to the support member, incubating the support member comprising the fluid sample and/or washing the support member, and

(d) an analysis section comprising a detection device for detecting an interaction between target and probe molecules, thereby analyzing analysing an interaction.

10. A method for the analysis of an interaction between target and probe molecules, comprising:

(a) administering a sample fluid possibly comprising target molecules to the support member of the device according to claim 1,

(b) entering the sample fluid into the channels of the support member by capillary forces or by applying a pressure difference over the support member, whereby the target molecules are contacted with the probe molecules,

(c) generating an alternating flow through the support member whereby at least part of the sample is forced to pass through the channels from the distal side of the support member to the proximal side of the support member and back at least one time, under conditions enabling the interaction between target and probe molecules, and

(d) analysing an interaction between target and probe molecules.