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Detection of nucleic acids from whole blood

  • US 20070161015A1
  • Filed: 10/04/2006
  • Published: 07/12/2007
  • Est. Priority Date: 10/05/2005
  • Status: Active Grant
First Claim
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1. A method of detecting at least a first target nucleic acid, the method comprising:

  • providing a sample comprising whole blood, which whole blood comprises peripheral blood cells;

    lysing the peripheral blood cells to produce a lysate, which lysate comprises the first target nucleic acid;

    contacting the first target nucleic acid with a first set of n capture extenders, wherein n is at least two, which first set of capture extenders is capable of hybridizing to the first target nucleic acid;

    hybridizing the first target nucleic acid to the first set of capture extenders;

    associating the first set of capture extenders with a solid support, whereby hybridizing the first target nucleic acid to the first set of capture extenders and associating the first set of capture extenders with the solid support captures the first target nucleic acid on the solid support; and

    detecting the presence of the first target nucleic acid on the solid support.

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