APPARATUS AND METHOD FOR HARVESTING AND HANDLING TISSUE SAMPLES FOR BIOPSY ANALYSIS

• US 20070166834A1
• Filed: 12/20/2006
• Published: 07/19/2007
• Est. Priority Date: 10/05/1998
• Status: Abandoned Application

• Current Assignee: Biopath Automation, LLC
• Original Assignee: Biopath Automation, LLC
• Inventors: Ward, Thomas, Williamson, Warren, Whitlatch, Stephen, DiNovo, Dominic, Allen, Douglas
• Application Number:
  US11/613,478
• Publication Number:
  US 20070166834A1
• Time in Patent Office: Days
• US Class Current: 436/174
• CPC Class Codes: G01N 1/286 involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising microtomes G01N1/06; pulverising in general B02C; mixing in general B01F
• CPC Class Codes: G01N 1/36 Embedding or analogous mounting of samples
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A sectionable cassette for use in a process for harvesting and handling tissue samples for biopsy analysis is disclosed. In the procedure, a tissue biopsy sample is placed on a tissue trapping and supporting material that can withstand tissue preparation procedures and which can be cut with a microtome. The tissue is immobilized on the material and the material and the tissue are held in the cassette and are subjected to a process for replacing tissue fluids with wax, and then the tissue and the supporting material are sliced for mounting on slides using a microtome. Harvesting devices and containers using the filter material are disclosed. An automated process is also disclosed. One embodiment has the tissue trapping and supporting material porous and another embodiment includes a tissue supporting material that is not easily microtomed.

Claims

1. A method for preparing one or more biopsy tissue samples for:

histological examination, comprising;

placing a tissue sample on a microtome sectionable support;

immobilizing the tissue sample on the support;

subjecting the microtome sectionable support and the tissue sample to a process for replacing fluid in the tissue sample with a hardenable material;

embedding the microtome sectionable support and the tissue sample in an additional quantity of the hardenable material;

curing the hardenable material into a block; and

slicing the block with a microtome into thin slices of the hardenable material, the microtome sectionable support, and the tissue sample.

2. The method of claim 1, wherein the embedding material is wax.

3. The method of claim 1, wherein the microtome sectionable support further comprises a bottom portion configured to hold the tissue sample and a lid, and the step of immobilizing the tissue sample further comprises:

closing the lid on top of the tissue sample to trap the tissue sample in a desired orientation.

4. The method of claim 1, wherein the microtome sectionable support is coupled to a frame prior to being subjected to the process for replacing fluid in the tissue sample with the hardenable material, and the method further comprises securing the frame in the microtome prior to slicing the block.

5. The method of claim 4, wherein prior to embedding the microtome sectionable support and the tissue sample in the additional quantity of the hardenable material, the microtome sectionable support is moved from a first position within the frame to a second position in which the support and tissue sample are exposed for sectioning in the microtome.

6. The method of claim 5, wherein the frame is configured to be manipulated by an automated machine, and further comprising:

using the automated machine to move the frame and the support from the first position to the second position.

7. The method of claim 1, wherein the microtome sectionable support is configured to be manipulated by an automated machine, and further comprising:

using the automated machine to perform the embedding step.

8. The method of claim 1, wherein the microtome sectionable support is configured to be manipulated by an automated machine, and further comprising:

using the automated machine to perform the curing step.

9. The method of claim 1, wherein the microtome sectionable support is configured to be manipulated by an automated machine, and further comprising:

automatically loading the support with the automated machine into a position for performing the embedding and curing steps; and

automatically unloading the support with the automated machine from the position for performing the embedding and curing steps upon completion of the embedding and curing steps.

10. An automated machine for preparing one or more tissue samples in a microtome sectionable support, comprising:

a loading device operating to place the microtome sectionable support into a position;

a dispensing device operating to dispense an embedding material onto both the microtome sectionable support and at least one tissue sample carried by the support; and

an unloading device operating to remove the microtome sectionable support after embedding of the tissue sample.

11. The automated machine of claim 10, further comprising:

a cooling device operative at said position for cooling and hardening the embedding material dispensed onto both the microtome sectionable support and the tissue sample.

12. The automated machine of claim 10, wherein the microtome sectionable support is received within a frame and is movable between a first position within the frame and a second position in which the embedded tissue sample is exposed for sectioning in a microtome, and the automated machine further comprises:

a setting head operative to move the support from the first position to the second position.

13. A method for preparing a tissue sample for analysis, comprising:

orienting the tissue sample in a desired orientation;

immobilizing the oriented tissue sample in a support structure having a porous top, porous bottom and porous sides surrounding the oriented tissue sample; and

processing the immobilized tissue sample with tissue processing fluid by allowing the tissue processing fluid to flow through the porous top, porous bottom and porous sides into the tissue sample.

14. The method of claim 13, wherein immobilizing the oriented tissue sample further comprises immobilizing the oriented tissue sample between the porous bottom and an opposing porous member.

15. The method of claim 13, further comprising:

sectioning the tissue sample and the support structure with the tissue sample in the desired orientation following processing.

16. A method for preparing a tissue sample for analysis, comprising:

orienting the tissue sample in a desired orientation relative to a porous support;

immobilizing the oriented tissue sample on the porous support using an immobilization media; and

processing the immobilized tissue sample with tissue processing fluid by allowing the tissue processing fluid to flow through the porous support into the tissue sample.

17. The method of claim 16, wherein the immobilization media further comprises a glue-like substance on at least one of the tissue sample and the porous support.

18. The method of claim 16, wherein the immobilization media further comprises agar.

19. A method for preparing a tissue sample for analysis, comprising:

orienting the tissue sample in a desired orientation;

immobilizing the oriented tissue sample on a porous support structure;

processing the immobilized tissue sample with tissue processing fluid by allowing the tissue processing fluid to flow through the porous support structure into the tissue sample; and

sectioning the tissue sample and the support structure with the tissue sample in the desired orientation following processing.

20. A method for embedding and sectioning a tissue sample comprising:

positioning the tissue sample above a planar, perforated element that is positioned on and forms a portion of an embedding support structure that has all portions of the planar, perforated element lying below the tissue sample, said planar perforated element defining a reference plane with respect to the tissue sample;

embedding the tissue sample and at least a portion of said support structure in an embedding medium to form an integral embedded unit with the tissue sample remaining above the planar, perforated element; and

,

sectioning the integral embedded unit in at least one plane that passes through the embedded tissue sample and is in registered relationship to said reference plane.

21. The method of claim 20, wherein said tissue sample is positioned on top of and in physical contact with said planar, perforated element.

22. The method of claim 20, wherein said tissue sample is positioned on top of, but not in physical contact with said planar, perforated element.

23. A method for embedding and sectioning a tissue sample for microtome sectioning comprising:

positioning the tissue sample above a planar, perforated element that is positioned on and forms a portion of support structure that has all portions thereof lying below the tissue sample, said planar perforated element defining a reference plane with respect to the tissue sample;

embedding the tissue sample and at least a portion of the support structure in an embedding medium to form an integral embedded unit with the tissue sample remaining above the planar, perforated element;

mounting the integral embedded unit on a microtome with the reference plane parallel to a cutting plane of the microtome; and

,

sectioning the embedded tissue sample with the microtome.

24. The method of claim 23, wherein said tissue sample is positioned on top of and in physical contact with said planar, perforated element.

25. The method of claim 23, wherein positioning the tissue sample above a planar, perforated element further comprises positioning the tissue sample on an immobilization media.

26. A method of automating a tissue sample processing and embedding procedure performed prior to histological examination, comprising:

providing at least one tissue sample support member having a machine readable portion,

electronically reading tracking information related to the tissue sample from the machine readable portion, and

storing the tracking information in a computer readable form.

27. The method of claim 26, wherein the tracking information further comprises a tracking number.

28. The method of claim 26, further comprising:

storing an electronic image of the tissue sample in a computer readable form.

29. The method of claim 28, wherein the electronic image further comprises a digital picture.