Parallel microarray hybridization

• US 20070172840A1
• Filed: 01/26/2006
• Published: 07/26/2007
• Est. Priority Date: 01/26/2006
• Status: Abandoned Application

• Current Assignee: Board of Regents of Oklahoma State University
• Original Assignee: Board of Regents of Oklahoma State University
• Inventors: Liu, Lin, Chen, Zhongming, Chen, Jiwang
• Application Number:
  US11/340,105
• Publication Number:
  US 20070172840A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

A glass substrate with multiple identical microarrays is provided, for example, for the identification of genes via nucleic acid hybridization. The multiarray substrate permits the analysis, in parallel, of several different samples on the same substrate and thus under the same conditions. Results obtained using the multiarray substrate are therefore less variable than those obtained with conventional techniques.

Claims

1. A hybridization system, comprising

a single glass substrate;

a plurality of microarrays, wherein said microarrays are separated from one another; and

binding entities for binding one or more substances in one or more samples, said binding entities forming at least a part of each of said plurality of microarrays.

2. The hybridization system of claim 1, wherein said glass substrate is a glass microscope slide.

3. The hybridization system of claim 1, wherein each of said microarrays in said plurality of microarrays includes identical binding entities.

4. The hybridization system of claim 1, wherein said binding entities include nucleic acid.

5. The hybridization system of claim 4, wherein said nucleic acid is DNA.

6. The hybridization system of claim 1, wherein said plurality of microarrays is attached to said single glass substrate by printing.

7. The hybridization system of claim 1, further comprising a barrier between each microarray in said plurality of microarrays.

8. A method of producing a hybridization system, comprising the step of

printing a plurality of microarrays on a single glass substrate, wherein said microarrays are separated from one another, and wherein at least a part of each of said microarrays includes one or more binding entities.

9. The method of claim 8, wherein said single glass substrate is a glass microscope slide.

10. The method of claim 8, wherein each of said plurality of microarrays includes identical binding entities.

11. The method of claim 8, wherein each of said binding entities include nucleic acid.

12. The method of claim 11, wherein said nucleic acid is DNA.

13. The method of claim 8, further comprising the step of forming a barrier between each microarray of said plurality of microarrays.

14. A method of comparing, on a single substrate, hybridization patterns of molecules in a plurality of samples, comprising the steps of

exposing each microarray of a plurality of microarrays formed on a single glass substrate and separated from one another to

a) one sample of said plurality of samples;

or

b) two or more samples of said plurality of samples, wherein said two or more samples are differentially labeled; and

detecting hybridization patterns of molecules in said plurality of samples.

15. The method of claim 14, wherein said single glass substrate is a glass microscope slide.

16. The method of claim 14, wherein said plurality of microarrays are identical.

17. The method of claim 14, wherein said plurality of microarrays comprise nucleic acid.

18. The method of claim 17, wherein said nucleic acid is DNA.

19. The method of claim 14, wherein said plurality of microarrays is attached to said single glass substrate by printing.

20. The method of claim 14, wherein a barrier if formed between each microarray in said plurality of microarrays.