Nucleic Acid Probes and Microarrays for Analysis of Polynucleotides
First Claim
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1. A probe for detecting a target polynucleotide, comprising:
- a target complementary region that base-pairs with said target polynucleotide to form a probe/target duplex;
a stem-complementary region bound to the target complementary region, anda RNA hairpin extension domain bound to the target complementary region via the stem-complementary region, wherein the RNA hairpin extension domain includes a stem-loop region.
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Abstract
The invention provides a probe for detecting a target polynucleotide. The probe contains a region that base-pairs with a target polynucleotide to form a duplex and a RNA hairpin extension domain that increases the stability of the duplex. The probe may further include a nucleotide clamp, a stem-complementary region and/or a linker moiety. Also provided is an array of subject probes bound to a surface of a solid support. Methods of using a subject probe to assess target polynucleotides, e.g., small RNAs, in a sample are provided, as are kits for use in practicing the subject methods.
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Citations
18 Claims
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1. A probe for detecting a target polynucleotide, comprising:
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a target complementary region that base-pairs with said target polynucleotide to form a probe/target duplex; a stem-complementary region bound to the target complementary region, and a RNA hairpin extension domain bound to the target complementary region via the stem-complementary region, wherein the RNA hairpin extension domain includes a stem-loop region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification