Methods for sequence determination using depolymerizing agent
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Abstract
A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
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Citations
55 Claims
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10. A composition comprising a depolymerizing agent including a molecular and/or atomic tag at or near the depolymerizing agent, associated with the depolymerizing agent or covalently bonded to a site on the depolymerizing agent and a depolymerizable polymer comprising a sequence of monomer types, where the tag has a detectable property that undergoes a change before, during and/or after each of a sequence monomer removals.
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37. A composition comprising:
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a depolymerizing agent modified including at least one pair of molecular and/or atomic tags located at or near the depolymerizing agent, associated with the depolymerizing agent, or covalently bonded to sites on the depolymerizing agent, and a depolymerizable polymer comprising a sequence of monomer types, where a detectable property of at least one tag of the pair undergoes a change before, during and/or after a sequence of monomer releases or where a detectable property of at least one tag of the pair undergoes a change before, during and/or after the sequence monomer releases due to a change in inter-tag interaction. - View Dependent Claims (38, 39, 40, 53)
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41. A composition comprising:
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a depolymerizing agent including a depolymerizing agent tag, and a depolymerizable polymer comprising a sequence of monomer types, each monomer type including a monomer tag, where a detectable property of at least one of the tags changes before, during and/or after each of a sequence of monomer releases due to an interaction between the depolymerizing agent tag and the monomer tag before, during and/or after each monomer release. - View Dependent Claims (42, 43, 44, 45, 46, 47, 48, 54)
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49. A composition comprising:
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an exonuclease including at least one molecular and/or atomic tag located at or near the exonuclease, associated with the exonuclease or covalently bonded to a site on the agent the exonuclease, and a depolymerizable polymer comprising a sequence of monomer types, where a detectable property of at least one of the tags undergoes a change before, during and/or after each of a sequence of monomer removals. - View Dependent Claims (50, 51, 55)
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Specification