Real time electronic cell sensing system and applications for cell-based assays
First Claim
1. A device for monitoring cell-substrate impedance, comprising:
- a) a nonconducting substrate;
b) two or more electrode arrays fabricated on the substrate, wherein each of said two or more electrode arrays comprises two electrode structures; and
c) at least two connection pads;
wherein for each of said two or more electrode arrays, each of said two electrode structures comprises multiple electrode elements and the first of said two electrode structures of each of said at least two electrode arrays is connected to one of said at least two connection pads, and the second of said two electrode structures of each of said at least two electrode arrays is connected to another of said at least two connection pads;
further wherein the electrode resistance at a first location on any one array of said two or more electrode arrays and the electrode resistance at a second location on said any one array do not differ by more than 30%; and
further wherein said substrate has a surface suitable for cell attachment or growth;
wherein said cell attachment or growth on said substrate can result in a detectable change in impedance between or among said electrode structures within each electrode array.
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Accused Products
Abstract
The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.
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Citations
51 Claims
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1. A device for monitoring cell-substrate impedance, comprising:
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a) a nonconducting substrate;
b) two or more electrode arrays fabricated on the substrate, wherein each of said two or more electrode arrays comprises two electrode structures; and
c) at least two connection pads;
wherein for each of said two or more electrode arrays, each of said two electrode structures comprises multiple electrode elements and the first of said two electrode structures of each of said at least two electrode arrays is connected to one of said at least two connection pads, and the second of said two electrode structures of each of said at least two electrode arrays is connected to another of said at least two connection pads;
further wherein the electrode resistance at a first location on any one array of said two or more electrode arrays and the electrode resistance at a second location on said any one array do not differ by more than 30%; and
further wherein said substrate has a surface suitable for cell attachment or growth;
wherein said cell attachment or growth on said substrate can result in a detectable change in impedance between or among said electrode structures within each electrode array. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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4. (canceled)
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51-159. -159. (canceled)
Specification