Reagent for immature leukocyte analysis and reagent kit
First Claim
1. A reagent for analyzing immature leukocyte contained in a sample, which reagent comprising a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte, a solubilizing agent for causing contraction to damaged blood cell, a sugar, and a dye for staining nucleic acid.
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Abstract
A reagent for analyzing immature leukocyte capable of analyzing accurately immature leukocyte such as myeloblast and immature granulocyte, and mature leukocyte, which reagent has broader allowable range of sample processing conditions than conventional ones, and a reagent kit are provided. The present invention relates to a reagent for analyzing immature leukocyte containing a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte in the sample, a solubilizing agent for causing contraction to damaged blood cell, sugar, a dye for staining nucleic acid.
29 Citations
20 Claims
- 1. A reagent for analyzing immature leukocyte contained in a sample, which reagent comprising a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte, a solubilizing agent for causing contraction to damaged blood cell, a sugar, and a dye for staining nucleic acid.
- 9. A reagent kit for analyzing immature leukocyte contained in a sample comprising a first reagent which comprises a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte, a solubilizing agent for causing contraction to damaged blood cell, and an osmotic pressure regulator, having osmotic pressure of from 150 to 600 mOsm/kg and electric conductivity of less than 6 mS/cm, and a second reagent containing a dye for staining nucleic acid.
- 15. A reagent kit for analyzing immature leukocyte contained in a sample comprising a first reagent which comprises a surfactant for giving damage to cell membrane of red blood cell and mature leukocyte, a solubilizing agent for causing contraction to damaged blood cell, sugar, a second reagent containing a dye for staining nucleic acid.
Specification