SURFACE MODIFIED PROTEIN MICROPARTICLES

• US 20070184119A1
• Filed: 04/10/2007
• Published: 08/09/2007
• Est. Priority Date: 06/17/2003
• Status: Abandoned Application

• Current Assignee: Daniel Marks, Farah Toublan, Kenneth Suslick, Stephen Boppart
• Original Assignee: Daniel Marks, Farah Toublan, Kenneth Suslick, Stephen Boppart
• Inventors: Marks, Daniel, Suslick, Kenneth, Boppart, Stephen, Toublan, Farah
• Application Number:
  US11/733,614
• Publication Number:
  US 20070184119A1
• Time in Patent Office: Days
• US Class Current: 424/491
• CPC Class Codes: A61K 49/001 Preparation for luminescence or biological staining

Abstract

A microparticle contains a cross-linked protein shell, and a surface coating.

Claims

1. (canceled)

2. (canceled)

4-6. -6. (canceled)

8. (canceled)

12. (canceled)

16. (canceled)

19-21. -21. (canceled)

22. (canceled)

23. (canceled)

27. (canceled)

29. (canceled)

30. (canceled)

33. A method of making a microparticle, comprising:

forming a cross-linked protein shell, and

attaching a surface coating to the cross-linked protein shell.

3. The method of claim 33, wherein the surface coating comprises polyethylene glycol.

7. The method of claim 33, wherein the surface coating comprises a second protein.

9. The method of claim 7, wherein the second protein is an antibody.

10. The method of claim 33, wherein the cross-linked protein shell comprises cross-linked albumin.

11. The method of claim 3, wherein the cross-linked protein shell comprises cross-linked albumin.

17. The method of claim 3, wherein the polyethylene glycol contains a ligand for a cell-surface receptor.

18. The method of claim 17, wherein the ligand is folate.

25. The method of claim 33, wherein the microparticle further comprises a core comprising a pharmaceutically active compound.

26. The microparticle method of claim 33, wherein the microparticle further comprises a core comprising a member selected from the group consisting of Gd complexes, melanin, colloidal gold, iron oxide, titanium dioxide, and fluorocarbons.

31. The method of claim 33, wherein the cross-linked protein shell comprises at least one cross-linked protein, the protein selected from the group consisting of hemoglobin, pepsin, immunoglobulin, lipase, peroxidase and myoglobin.

34. A method of making a microparticle, comprising:

forming a cross-linked protein shell, and

attaching a surface coating to the cross-linked protein shell,

wherein the surface coating comprises particles.

13. The method of claim 34, wherein the cross-linked protein shell comprises cross-linked albumin.

14. The method of claim 34, wherein the particles are colloidal particles selected from the group consisting of ceramics, metals and semiconductors.

15. The method of claim 34, wherein the particles are selected from the group consisting of melanin, carbon, gold, and silica.

28. The method of claim 34, wherein the microparticle further comprising a core comprising a member selected from the group consisting of Gd complexes, melanin, colloidal gold, iron oxide, titanium dioxide, and fluorocarbons.

32. The method of claim 34, wherein the cross-linked protein shell comprises at least one cross-linked protein, the protein selected from the group consisting of hemoglobin, pepsin, immunoglobulin, lipase, peroxidase and myoglobin.

35. The method of claim 34, wherein the microparticle has a surface charge and the particles comprise colloidal particles, said colloidal particles have a charge opposite the surface charge.

36. A method of making a microparticle, comprising:

mixing an aqueous solution of a protein with particles suspended in oil, to form a mixture; and

irradiating the mixture with ultrasound to cross-link the protein and form the microparticle.

24. The method of claim 36, wherein the particles comprise melanin, carbon or gold.