Microparticle based biochip systems and uses thereof
First Claim
Patent Images
1. A device for analyzing an analyte, which device comprises:
- a) a controllably closed space enclosed by a suitable material on a substrate, wherein said suitable material is thermoconductive, biocompatible and does not inhibit binding between an analyte and a reactant, and said controllably closed space comprising, on the surface of said substrate, a first immobilized reactant capable of binding to said analyte;
b) a first means for controllably moving said analyte to said first immobilized reactant;
c) a second means for controllably moving said analyte to a labeled unimmobilized complex comprising a second reactant capable of binding to said analyte and a microparticle; and
d) a third means for controllably moving said labeled unimmobilized complex unbound to said analyte away from said first immobilized reactant, and wherein addition of a sample comprising said analyte and said labeled unimmobilized complex into said controllably closed space and operation of said means result in formation of a sandwich of said labeled unimmobilized complex-said analyte-said first immobilized reactant on said substrate.
1 Assignment
0 Petitions
Accused Products
Abstract
This invention relates generally to the field of analyte assays. In particular, the invention provides a device for analyzing an analyte, which device comprises, inter alia, various means for moving analytes and other items to facilitate binding between analytes and their binding reagents immobilized on a surface and to facilitate clearance of undesirable items away from analyte-binding reagent interaction area to reduce background noise in the assay. Methods for analyzing an analyte using the devices are also disclosed.
51 Citations
46 Claims
-
1. A device for analyzing an analyte, which device comprises:
-
a) a controllably closed space enclosed by a suitable material on a substrate, wherein said suitable material is thermoconductive, biocompatible and does not inhibit binding between an analyte and a reactant, and said controllably closed space comprising, on the surface of said substrate, a first immobilized reactant capable of binding to said analyte;
b) a first means for controllably moving said analyte to said first immobilized reactant;
c) a second means for controllably moving said analyte to a labeled unimmobilized complex comprising a second reactant capable of binding to said analyte and a microparticle; and
d) a third means for controllably moving said labeled unimmobilized complex unbound to said analyte away from said first immobilized reactant, and wherein addition of a sample comprising said analyte and said labeled unimmobilized complex into said controllably closed space and operation of said means result in formation of a sandwich of said labeled unimmobilized complex-said analyte-said first immobilized reactant on said substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
-
Specification