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Fret process

  • US 20070184453A1
  • Filed: 10/01/2003
  • Published: 08/09/2007
  • Est. Priority Date: 10/02/2002
  • Status: Abandoned Application
First Claim
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1. A pair of FRET hybridization probes hybridizing adjacently to a target nucleic acid sequence, a first member of said pair of hybridization probes comprising:

  • a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid;

    a fluorescent entity, said entity being either a FRET donor entity or a FRET acceptor entity; and

    a spacer entity connecting said nucleotide sequence entity and said fluorescent entity, said spacer entity comprising a connecting chain of at least 15 atoms, wherein 2 atoms of said connecting chain of at least 15 atoms comprise negatively charged substituents.

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