Microfluidic device for purifying a biological component using magnetic beads

• US 20070184463A1
• Filed: 10/02/2006
• Published: 08/09/2007
• Est. Priority Date: 09/30/2005
• Status: Abandoned Application

• Current Assignee: Canon US Life Sciences Inc (Canon Inc.), Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Canon US Life Sciences Inc (Canon Inc.), Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Foreman, Pamela, Molho, Josh
• Application Number:
  US11/542,652
• Publication Number:
  US 20070184463A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: G01N 1/34 Purifying; Cleaning processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C

Abstract

A method of purifying a biological component found in a biological sample by extracting the biological component from the biological sample. The method is performed using a microfluidic device having at least one well for receiving the biological sample and at least one channel for introducing and removing fluids. A plurality of magnetic beads having a factor with an affinity for the biological component is introduced to the well together with a suitable biological sample. The biological sample is manipulated to release the biological component in proximity to the magnetic beads which are then segregated within the well while removing the biological sample. An elution solution for the biological component is introduced to the well and the elution solution together with the biological component are withdrawn therefrom.

Claims

1. A method of purifying a biological component found in a biological sample by extracting said biological component from said biological sample, said method being performed in a microfluidic device having at least one well for receiving said biological sample and at least one channel for introducing and removing fluids in performing the present method, said method comprising providing a plurality of magnetic beads having a factor with an affinity for said biological component, introducing said magnetic beads into said well together with said biological sample, manipulating said biological sample to release said biological component in proximity to said magnetic beads, magnetically segregating said magnetic beads within said well, removing said biological sample from said well, introducing an elution solution for said biological component into said well and removing said elution solution together with said biological component from said well.

2. The method of claim 1 wherein said biological sample is washed from said magnetic beads coated with said biological component prior to the introduction of said elution solution.

3. The method of claim 1 wherein said factor and said biological component comprise DNA.

4. The method of claim 1 wherein a magnet applied to the exterior of said well causes said magnetic beads to segregate to a side wall of said well.

5. The method of claim 1 wherein said biological sample is removed from said well after said biological sample has been manipulated to release said biological component by introducing and removing a wash liquid through said well.

6. The method of claim 5 wherein said magnetic beads are agitated when said wash liquid is in contact with said magnetic beads.

7. The method of claim 1 wherein said magnetic beads are agitated when said elution solution is in contact with said magnetic beads.

8. The method of claim 1 wherein said magnetic beads are magnetically manipulated to aggregate proximate said at least one channel used to introduce said elution solution during the practice of said method when said elution solution is fed into said well.

9. The method of claim 8 wherein said magnetic beads are magnetically manipulated to be removed from an area proximate said at least one channel when said elution solution is removed from said well through said at least one channel.

10. The method of claim 1 wherein an electric field is applied to said magnetic beads when said elution solution is in contact with said magnetic beads.

11. A method of purifying a biological component found in a biological sample by extracting said biological component from said biological sample, said method being performed in a microfluidic device having a well for receiving said biological sample and a channel for introducing and withdrawing fluids into and from said well, said method comprising providing a plurality of magnetic beads having a factor with an affinity for said biological component, introducing said magnetic beads to said well together with said biological sample, manipulating said biological sample to release said biological component in proximity to said magnetic beads, magnetically segregating said magnetic beads proximate said channel, introducing a wash liquid to said well through said channel for washing said biological sample from said magnetic beads and maintaining a volume of wash liquid between said magnetic beads and said biological sample, introducing an elution solution for said biological component from said channel, said elution solution residing in proximity to said wash liquid and spaced from said biological sample, and removing said elution solution and biological component from said well through said channel.

12. The method of claim 11 wherein said factor and said biological component comprise DNA.

13. The method of claim 11 wherein a magnetic force applied to the exterior of said well segregates said magnetic beads to a sidewall of said well.

14. The method of claim 11 wherein said magnetic beads are agitated when said elution solution is in contact with said magnetic beads.

15. The method of claim 11 wherein said magnetic beads are magnetically manipulated to be removed from an area proximate said channel when said elution solution is removed from said well through said channel.

16. A method of purifying a biological component found in a biological sample by extracting said biological component from said biological sample, said method being performed in a microfluidic device comprising a well for receiving said biological sample and a first and a second channel for introducing and withdrawing fluids into and from said well, said method comprising providing a plurality of magnetic beads having a factor with an affinity for said biological component, introducing said magnetic beads to said well together with said biological sample, manipulating said biological sample to release said biological component in proximity to said magnetic beads, magnetically segregating said magnetic beads proximate said first channel, introducing a wash liquid through said first channel and drawing said wash liquid and biological sample from said well through said second channel, introducing an elution solution for said biological sample through said first channel in proximity to said magnetic beads, and removing said elution solution together with said biological component from said well through said first channel.

17. The method of claim 16 wherein said factor and said biological component comprise DNA.