Dendritic chemiluminescent substrates
First Claim
1. A chemiluminescent substrate delivery system comprising:
- a dendrimer;
and at least one enzymatically activated chemiluminescent substrate conjugated to the dendrimer.
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Accused Products
Abstract
Chemiluminescent substrate delivery systems comprising a conjugate a dendrimer and at least one chemiluminescent substrate are provided. The substrate delivery systems can also include a chemiluminescence enhancer. The dendrimer/chemiluminescent substrate conjugates can be used in kits including an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. The dendrimer/chemiluminescent substrate conjugates can be used in assays to detect the presence of an analyte (e.g., an enzyme, an antibody, an antigen or a nucleic acid) in a sample.
14 Citations
52 Claims
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1. A chemiluminescent substrate delivery system comprising:
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a dendrimer;
and at least one enzymatically activated chemiluminescent substrate conjugated to the dendrimer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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8-15. -15. (canceled)
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34. A kit for conducting an assay to determine the presence or concentration of an analyte in a sample, wherein the kit comprises:
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a dendrimer comprising at least one enzymatically active chemiluminescent substrate moiety; and
an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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46. A method for detecting the presence of an analyte in a sample comprising:
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forming an enzyme complex between an enzyme and a substance capable of binding to the analyte;
adding the enzyme complex to the sample;
allowing the enzyme complex to bind with analyte present in the sample;
adding a chemiluminescent delivery system to the sample; and
measuring chemiluminescent emissions from the sample;
wherein the chemiluminescent delivery system comprises a dendrimer and at least one enzymatically active chemiluminescent substrate conjugated to the dendrimer and wherein the amount of chemiluminescent emissions measured indicates the presence and/or the concentration of the analyte in the sample. - View Dependent Claims (47, 48, 49, 50, 51, 52)
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Specification