Polynucleotide sample preparation device
First Claim
1. A microfluidic system for converting a sample containing one or more polynucleotides into a form suitable for analyzing the one or more polynucleotides, the system comprising:
- a cartridge receiving element in communication with an insertable and removable cartridge;
a heating element in communication with the cartridge receiving element, configured to heat one or more regions of the cartridge; and
control circuitry in communication with the heating element;
wherein the insertable cartridge comprises;
at least one microfluidic component that, in conjunction with the heating element and the control circuitry, is configured to accept the sample and one or more reagents, and to react the sample and the reagents, in order to produce a prepared sample suitable for analysis of the one or more polynucleotides.
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Accused Products
Abstract
Methods and systems for preparing polynucleotide samples are disclosed. The invention includes a microfluidic system for converting a sample containing one or more polynucleotides into a form suitable for analyzing the polynucleotides, comprising: a cartridge receiving element, an insertable and removable cartridge, a heating element configured to heat one or more regions of the cartridge, and control circuitry, wherein the insertable cartridge comprises: a microfluidic component that is configured to accept the sample and one or more reagents, and to react the sample and the reagents, in order to produce a prepared sample suitable for analyzing the one or more polynucleotides. The invention further comprises a multi-sample cartridge for converting a number of samples, each containing one or more polynucleotides, into respective forms suitable for analyzing the polynucleotides, comprising: at least a first microfluidic component and a second microfluidic component.
280 Citations
38 Claims
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1. A microfluidic system for converting a sample containing one or more polynucleotides into a form suitable for analyzing the one or more polynucleotides, the system comprising:
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a cartridge receiving element in communication with an insertable and removable cartridge;
a heating element in communication with the cartridge receiving element, configured to heat one or more regions of the cartridge; and
control circuitry in communication with the heating element;
wherein the insertable cartridge comprises;
at least one microfluidic component that, in conjunction with the heating element and the control circuitry, is configured to accept the sample and one or more reagents, and to react the sample and the reagents, in order to produce a prepared sample suitable for analysis of the one or more polynucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 19, 20, 21, 22, 23, 24, 25, 38)
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17. A microfluidic cartridge for converting a sample containing one or more polynucleotides into a form suitable for analyzing the one or more polynucleotides, the cartridge comprising:
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a sample inlet for receiving the sample;
a reagent inlet for accepting one or more reagents;
an outlet for directing prepared sample into a PCR tube; and
a microfluidic component having one or more channels configured to transmit volumes of fluid in the range 0.1-50 μ
l;
wherein the one or more channels ensure passage of sample, reagents, and fluid between the sample inlet, the reagent inlet, and the outlet; and
wherein the microfluidic cartridge, in conjunction with an external source of heat, is configured to react the sample and the reagents, in order to produce a prepared sample suitable for analyzing the one or more polynucleotides. - View Dependent Claims (18)
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26. A method of converting a sample comprising a number of cells that have one or more polynucleotides into a form suitable for analyzing the one or more polynucleotides, the method comprising:
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introducing from about 0.1-2.0 mL of the sample and an excess quantity of air into a bulk lysis chamber;
applying heat to the sample in the bulk lysis chamber, to raise the sample to a first temperature, thereby lysing cells in the sample and producing a lysate containing the one or more polynucleotides;
capturing one or more polynucleotides in the lysate on an affinity matrix;
causing the beads to leave the bulk lysis chamber and be trapped on a filter;
washing the beads with a wash reagent;
displacing the wash reagent with a release buffer;
heating the beads to a second temperature, thereby releasing the one or more polynucleotides; and
causing the one or more polynucleotides to be transferred to a PCR tube. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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Specification