Molecularly imprinted polymers selective for nitrosamines and methods of using the same
First Claim
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1. A molecularly imprinted polymer selective for at least one tobacco-specific nitrosamine, wherein said molecularly imprinted polymer is prepared using a tobacco-specific nitrosamine template or an isosteric analogue thereof.
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Abstract
A class of molecularly imprinted polymers that specifically recognizes and binds to nitrosamines members of which class are useful, for example, in analysis and separation of nitrosamines from biological fluids. Such molecularly imprinted polymers are also useful in methods of treating and manufacturing tobacco products and materials.
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Citations
26 Claims
- 1. A molecularly imprinted polymer selective for at least one tobacco-specific nitrosamine, wherein said molecularly imprinted polymer is prepared using a tobacco-specific nitrosamine template or an isosteric analogue thereof.
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7. A kit, comprising:
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a molecularly imprinted polymer selective for at least one tobacco-specific nitrosamine, wherein the polymer is prepared using a tobacco-specific nitrosamine template or an isosteric analogue thereof; and
instructions for using the polymer to perform at least one of detecting, quantifying, and separating nitrosamines in a sample.
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8. A method of preparing a molecularly imprinted polymer, comprising:
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co-polymerizing at least one functional monomer and at least one cross-linker in the presence of at least one tobacco-specific nitrosamine template or an isosteric analogue thereof in a polymerization medium containing at least one free radical initiator to produce the molecularly imprinted polymer bound to the tobacco-specific nitrosamine template or the isosteric analogue thereof; and
removing the template or the isosteric analogue thereof from the molecularly imprinted polymer. - View Dependent Claims (9, 10, 11, 12)
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13. A method of preparing a molecularly imprinted polymer comprising:
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co-polymerizing a polymerization mixture and at least one tobacco-specific nitrosamine template or an isosteric analogue thereof in a homogeneous reaction medium; and
recovering suspended particles from the reaction medium.
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14. A method of preparing a molecularly imprinted polymer comprising:
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co-polymerizing a polymerization mixture and at least one tobacco-specific nitrosamine template or an isosteric analogue thereof in a heterogeneous reaction medium; and
recovering polymerized material from the heterogeneous reaction medium.
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15. A method of determining if a sample of a tobacco product contains nitrosamines, comprising:
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reacting the sample with a molecularly imprinted polymer under conditions that would allow binding of nitrosamines present in the sample to the molecularly imprinted polymer; and
evaluating whether the molecularly imprinted polymer has bound to any nitrosamines, wherein an evaluation resulting in observation of molecularly imprinted polymer bound to nitrosamines indicates that the sample contains nitrosamines.
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16. A method of quantifying the amount of nitrosamines in a sample of a tobacco product, comprising:
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reacting the sample with a molecularly imprinted polymer under conditions that would allow binding of nitrosamines present in the sample to the molecularly imprinted polymer; and
measuring the amount of nitrosamines bound to the molecularly imprinted polymer.
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- 17. A method of treating a tobacco product to reduce the level of a targeted component therein comprising treating the tobacco product with a molecularly imprinted polymer.
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22. A method of manufacturing a tobacco material, comprising the steps of:
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extracting nitrosamines from a material containing tobacco, a tobacco substitute or a mixture thereof by contacting said material with a solvent to produce an extract;
contacting the extract containing nitosamine with a molecularly imprinted polymer to reduce the level of nitorsamines in the extract.
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23. A smoking article comprising:
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a smoking material; and
a molecularly imprinted polymer found in thermal decomposition products of the smoking material. - View Dependent Claims (24)
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- 25. A smoke filter comprising a molecularly imprinted polymer selective for at least one tobacco-specific nitrosamine.
Specification