Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
First Claim
Patent Images
1. An analytical device, comprising:
- a substrate;
a plurality of signal sources disposed upon the substrate, the signal sources being arrayed upon the substrate in a plurality of substantially parallel rows, each of the plurality of parallel rows comprising a plurality of signal sources, and wherein two adjacent signal sources in a row are spaced apart by a first distance, and wherein two adjacent rows of signal sources are spaced apart by a second distance, wherein the second distance is at least three times greater than the first distance.
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Abstract
Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.
180 Citations
28 Claims
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1. An analytical device, comprising:
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a substrate; a plurality of signal sources disposed upon the substrate, the signal sources being arrayed upon the substrate in a plurality of substantially parallel rows, each of the plurality of parallel rows comprising a plurality of signal sources, and wherein two adjacent signal sources in a row are spaced apart by a first distance, and wherein two adjacent rows of signal sources are spaced apart by a second distance, wherein the second distance is at least three times greater than the first distance. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. An analytical system, comprising:
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a substrate having a plurality of discrete signal sources disposed thereon; an excitation light source; an optical train positioned to transmit excitation light from the excitation light source to the substrate to illuminate a first plurality of illuminated signal sources and a second plurality of illuminated signal sources, and image signals from the pluarality of illuminated signal sources onto an array detector; wherein one or more of the substrate and the optical train are configured such that the first and second plurality of illuminated signal sources is spaced from the other by a first distance of that is at least three times a cross sectional dimension of an image of a signal from a signal source imaged onto the array detector. - View Dependent Claims (9, 10, 11)
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12. A method of analyzing a plurality of signal sources, comprising:
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providing a substrate having a plurality of discrete signal sources disposed thereon; illuminating a first plurality of illuminated signal sources and a second plurality of illuminated signal sources, wherein the first plurality of illuminated signal sources are spaced apart from the second plurality of illuminated signal sources by a distance that is greater than three times a cross sectional dimension of an image of the signal source imaged on a detector array; and imaging the signal source on a detector array. - View Dependent Claims (13, 14, 15, 16, 17)
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18. An analytical system, comprising:
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a substrate having a plurality of signal sources disposed thereon; an excitation light source; an optical train configured to; receive excitation light from the excitation light source; direct it onto the substrate in at least first and second substantially parallel linear illumination profiles, wherein the first and second linear illumination profiles are spaced apart on the substrate by a distance that is at least two times a width of the first linear illumination profile; and receive optical signals from the substrate an image the oiptical signals onto a detector array.
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19. An analytical system, comprising:
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a substrate comprising a plurality of discrete signal sources disposed thereon, wherein at least a first subset of signal sources are positioned in a first substantial linear orientation, and a second subset of signal sources are positioned in a second substantially linear orientation that is substantially parallel to the first linear orientation; an light source; an optical train for directing light from the light source to the substrate in at least first and second substantially parallel linear illumination profiles, the first linear profile illuminating the first subset of signal sources and the second illumination profile illuminating the second subset of signal sources. - View Dependent Claims (20, 21)
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22. A method of detecting fluorescent signals from a plurality of signal sources on a substrate, comprising directing excitation radiation at portions of the substrate occupied the plurality of signal sources on a substrate while not directing excitation radiation at portions of the substrate not occupied by the signal sources.
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23. A system, comprising:
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a substrate comprising a plurality of discrete signal sources; an excitation light source; an optical train, positioned to receive excitation light from the excitation light source and direct the excitation light to the substrate, wherein the optical train is configured to direct excitation light in a substantially linear illumination profile at a plurality of signal sources, simultaneously, and simultaneously receive optical signals from the plurality of signal sources and direct the optical signals upon an imaging detector, to detect the optical signals from the plurality of signal sources.
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24. A system, comprising:
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a substrate having at least first and second rows of signal sources disposed thereon; an excitation light source; and an optical train, positioned to receive excitation light from the excitation light source and direct the excitation light to the substrate, wherein the optical train is configured to divide the excitation light into at least first and second discrete beams, and direct each of the at least first and second discrete beams in a substantially linear illumination profile at the substrate, wherein the first beam simultaneously illuminates a plurality of signal sources in the first row of signal sources, and the second beam simultaneously illuminates a plurality of signal sources in the second row of signal sources.
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25. A system, comprising:
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an excitation light source; an optical train positioned to receive excitation light from the excitation light source and direct excitation light to the substrate, the optical train comprising; a polarizing beam splitter to split the excitation light into at least first and second polar component beams; and optical components for directing each of the first and second polar component beams to different locations on the substrate.
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26. A method of analyzing a plurality of signal sources on a substrate, comprising:
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providing at least first and second adjacent signal sources on a substrate; selectively directing excitation radiation at the first and second signal sources while not substantially illuminating space between the first and second signal sources. - View Dependent Claims (27, 28)
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Specification