Capsule and tray systems for combined sample collection, archiving, purification, and PCR
First Claim
1. A method for manipulating at least one biological sample, said method comprising:
- providing a biological sample purification device comprising a tubular body having a first end, a first end opening, a second end, and a second end opening, and a species-immobilizing filter within the tubular body;
introducing a biological sample into the tubular body through at least one of the first end opening and the second end opening of the tubular body;
causing a pressure differential across said species-immobilizing filter to immobilize a target analyte, if present in said sample, on or in said filter;
after said causing a pressure differential, sealing at least one of said first and second end openings with a sealing device, said sealing device having a surface adapted to seal at least one of the first and second end openings of a plurality of said biological sample purification devices, to form a sealed device; and
archiving said biological sample purification device.
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Accused Products
Abstract
The present invention relates to a biological sample collection, archiving, purification, and manipulating system and methods for collecting, archiving, purifying, and manipulating biological samples. The system can include a plurality of devices, each having a species-immobilizing filter within a tubular body. The body includes first and second end openings and one or more removable caps or sealing devices for sealing the end openings. A plurality of the devices can be positioned in an array tray for simultaneously archiving, purifying, or reacting respective samples collected in the respective devices. In another embodiment, a plate system is provided that includes a plate having a plurality of through-holes with each through-hole having a species-immobilizing filter disposed therein. The plate system also includes sealing devices such as sealing trays or removable caps for sealing first and second end openings of each through-hole of the plate.
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Citations
36 Claims
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1. A method for manipulating at least one biological sample, said method comprising:
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providing a biological sample purification device comprising a tubular body having a first end, a first end opening, a second end, and a second end opening, and a species-immobilizing filter within the tubular body;
introducing a biological sample into the tubular body through at least one of the first end opening and the second end opening of the tubular body;
causing a pressure differential across said species-immobilizing filter to immobilize a target analyte, if present in said sample, on or in said filter;
after said causing a pressure differential, sealing at least one of said first and second end openings with a sealing device, said sealing device having a surface adapted to seal at least one of the first and second end openings of a plurality of said biological sample purification devices, to form a sealed device; and
archiving said biological sample purification device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for archiving at least one biological sample, said method comprising:
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providing a biological sample purification device, said device comprising a tubular body having a first end, a first end opening, a second end, and a second end opening, and a species-immobilizing filter within the tubular body between said first end opening and said second end opening;
introducing a biological sample into the tubular body through at least one of the first end opening and the second end opening;
sealing at least one of said first and second end openings with a sealing device, such that both end openings are sealed to form a sealed device, said sealing device having a surface adapted to seal at least one respective end opening of each of a plurality of said devices; and
archiving the sealed device. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23)
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24. A method for separating at least one target analyte comprising a nucleic acid or nucleic acid fragment, from a biological sample, said method comprising:
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providing a nucleic acid or nucleic acid fragment sample purification device, said device comprising a tubular body having a first end, a first end opening, a second end, a second end opening, and a species-immobilizing filter within the tubular body;
introducing a biological sample including a nucleic acid or nucleic acid fragment through an end opening of the tubular body;
causing target analyte, if present in said sample, to be immobilized on or in said species-immobilizing filter;
removing components of said biological sample other than said target analyte, from said species-immobilizing filter; and
sealing at least one of said first and second end openings with a sealing device, such that both end openings are sealed to form a sealed device, said sealing device having a surface adapted to seal at least one respective end opening of each of a plurality of said purification devices. - View Dependent Claims (25, 26, 27, 28, 29)
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30. A method of manipulating a biological sample comprising:
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collecting a biological sample in or on one or more of a plurality of species-immobilizing filters of a system, the system comprising an analytical system for manipulating biological samples, the analytical system comprising a plate having a first surface and a second surface that opposes said first surface, and a plurality of through-holes, each through-hole extending from said first surface to said second surface and defining a first end opening at said first surface and a second end opening at said second surface, a plurality of species-immobilizing filters, each disposed within a respective one of said through-holes, and a first sealing device having a surface adapted to individually seal each first end opening of said plurality of through-holes during the manipulation of biological samples, the first sealing device comprising a tray and the tray comprising a plurality of recesses; and
then sealing the second end openings with a second sealing device adapted to seal each second end opening of said plurality of through-holes. - View Dependent Claims (31)
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32. A method comprising:
- introducing a biological sample including a target analyte in a first through-hole of a system, the system comprising
a plate having a first surface and a second surface that opposes said first surface, and a plurality of through-holes, each through-hole extending from said first surface to said second surface and defining a first end opening at said first surface and a second end opening at said second surface, a plurality of species-immobilizing filters, each disposed within a respective one of said through-holes, a first sealing device having a surface adapted to individually seal each first end opening of said plurality of through-holes during the manipulation of biological samples, the first sealing device comprising a tray and the tray comprising a plurality of recesses, and a second sealing device adapted to seal each second end opening of said plurality of through-holes, such that said target analyte is immobilized in or on the respective species-immobilizing filter within the first through-hole;
introducing a solution into at least said first through-hole, said solution having sufficient components to enable a reaction of the target analyte; and
subsequent to introducing said sample and said solution, sealing the first end openings and second end openings of said system to form a sealed system. - View Dependent Claims (33, 34)
- introducing a biological sample including a target analyte in a first through-hole of a system, the system comprising
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35. A method of archiving a biological sample, comprising:
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introducing a biological sample into one or more through-holes of a plurality of through-holes of a system, the system comprising a plate having a first surface and a second surface that opposes said first surface, and a plurality of through-holes, each through-hole extending from said first surface to said second surface and defining a first end opening at said first surface and a second end opening at said second surface, a plurality of species-immobilizing filters, each disposed within a respective one of said through-holes, a first sealing device having a surface adapted to individually seal each first end opening of said plurality of through-holes during the manipulation of biological samples, the first sealing device comprising a tray and the tray comprising a plurality of recesses, and a second sealing device adapted to seal each second end opening of said plurality of through-holes;
sealing the first end openings and the second end openings of said system to form a sealed device; and
archiving said sealed device. - View Dependent Claims (36)
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Specification