Microarray substrate, methods of manufacturing and use

• US 20070190541A1
• Filed: 09/19/2006
• Published: 08/16/2007
• Est. Priority Date: 12/20/2005
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Park, Jong-myeon, Peak, Sang-hyun
• Application Number:
  US11/523,459
• Publication Number:
  US 20070190541A1
• Time in Patent Office: Days
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A microarray substrate comprising a functionalized (poly)ethyleneglycol compound coated on a solid support having a polyanhydride surface is disclosed. Methods of manufacturing and using the substrate are also disclosed.

Claims

1. A microarray substrate comprising a functionalized (poly)ethyleneglycol compound coated on a solid support having a surface modified with a polyanhydride.

2. The microarray substrate of claim 1, wherein the (poly)ethyleneglycol compound is functionalized with an amino group, a thiol group, a hydroxy group, an epoxy group, or an ester group.

3. The microarray substrate of claim 2, wherein the (poly)ethyleneglycol compound is functionalized with the amino group.

4. The microarray substrate of claim 1, wherein the (poly)ethyleneglycol compound is a star-like or linear molecule.

5. The microarray substrate of claim 4, wherein the (poly)ethyleneglycol compound has a molecular weight of 60 to 10,000,000.

6. The microarray substrate of claim 1, wherein the polyanhydride is poly(ethylene-alt-maleic anhydride).

7. The microarray substrate of claim 6, wherein the poly(ethylene-alt-maleic anhydride) has a molecular weight of 128 to 10,000,000.

8. The microarray substrate of claim 1, wherein the solid support is made of a material selected from the group consisting of silicone, glass, and a plastic material.

9. The microarray substrate of claim 8, wherein the surface of the solid support is coated with an amino group-containing compound, a thiol group, or a hydroxyl group-containing compound.

10. The microarray substrate of claim 9, wherein the surface is coated with the amino group-containing compound;

and the amino group-containing compound is gamma-aminopropyltriethoxysilane, gamma-aminopropyldiethoxysilane, or aminohexyl.

11. The microarray substrate of claim 10, wherein the amino group-containing compound is gamma-aminopropyltriethoxysilane.

20. A microarray comprising a probe biomolecule immobilized on the microarray substrate of claim 1.

21. The microarray of claim 20, wherein the probe biomolecule is a nucleic acid.

22. A method of analyzing a target biomolecule, comprising

binding a target biomolecule to the probe molecule of the microarray of claim 20.

12. A method of manufacturing a microarray substrate, the method comprising:

(a) obtaining a solid support comprising a surface comprising an amino group-containing compound;

(b) immobilizing a polyanhydride on the surface by reacting the polyanhydride with the amino group-containing compound; and

(c) coating the polyanhydride-immobilized surface with a functionalized (poly)ethyleneglycol such that the functionalized (poly)ethyleneglycol reacts with the immobilized polyanhydride.

13. The method of claim 12, further comprising functionalizing an end of a (poly)ethyleneglycol with an amino group.

14. The method of claim 12, wherein the functionalized (poly)ethyleneglycol is a star-like or linear molecule.

15. The method of claim 12, wherein the functionalized (poly)ethyleneglycol has a molecular weight of 60 to 10,000,000.

16. The method of claim 12, wherein the polyanhydride is poly(ethylene-alt-maleic anhydride).

17. The method of claim 16, wherein the poly(ethylene-alt-maleic anhydride) has a molecular weight of 128 to 10,000,000.

18. The method of claim 12, wherein the solid support is made of a material selected from the group consisting of silicone, glass, and a plastic material.

19. The method of claim 12, wherein the amino group-containing compound is gamma-aminopropyltriethoxysilane, gamma-aminopropyldiethoxysilane, or aminohexyl.