BIOLOGICAL MATERIAL DETECTION ELEMENT, BIOLOGICAL MATERIAL DETECTION METHOD AND APPARATUS, CHARGED MATERIAL MOVING APPARATUS

• US 20070190665A1
• Filed: 03/26/2007
• Published: 08/16/2007
• Est. Priority Date: 08/31/2001
• Status: Abandoned Application

• Current Assignee: Akihiro Kasahara, Yoshio Ishimori
• Original Assignee: Akihiro Kasahara, Yoshio Ishimori
• Inventors: KASAHARA, Akihiro, ISHIMORI, Yoshio
• Application Number:
  US11/690,873
• Publication Number:
  US 20070190665A1
• Time in Patent Office: Days
• US Class Current: 436/514
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream

Abstract

A biological material detection apparatus which detects a charged biological material such as a gene or protein contained in a sample liquid is disclosed. A biological material detection element includes a substrate, at least one first electrode formed on the substrate, and a plurality of second electrodes which are arrayed at predetermined intervals around the first electrode on the substrate along the circumferential direction and to which ligands that react with predetermined biological materials are respectively immobilized. A sample liquid is introduced toward the first electrode on the substrate. The introduced sample liquid is moved radially toward the second electrodes by electrical control.

Claims

1-17. -17. (canceled)

18. A biological material moving apparatus for moving a charged biological material contained in a sample liquid, comprising:

a biological material detection element having a substrate and a plurality of electrodes which are arrayed at predetermined intervals along a circumferential direction on the substrate and to which ligands that react with predetermined biological materials are respectively immobilized;

a sample liquid introduction part to introduce the sample liquid to a central portion of the array of the electrodes on the substrate;

a sample liquid moving mechanism to move the sample liquid introduced to the central portion on the substrate by the sample liquid introduction part radially toward the electrodes by an electrostatic attracting force produced by the electrodes; and

a control device configured to control a process which is performed repeatedly, the process including

(a) supplying an intercalating agent onto the biological material detection element,

(b) measuring an electrochemical signal from the intercalating agent which is based on a reaction between the charged biological material and the ligand, and

(c) removing the intercalating agent adhering to the ligand.

19. A biological material moving apparatus to which a sample liquid containing a charged biological material is introduced, and which moves the biological material, comprising:

a biological material detection element having a substrate, at least one first electrode placed at a position on the substrate to which the sample liquid is introduced, and a plurality of second electrodes which are arrayed at predetermined intervals around the first electrode on the substrate along a circumferential direction, and to which ligands that react with predetermined biological materials are respectively immobilized;

a driving circuit which drives the biological material detection element by performing a driving operation of applying a voltage having the same polarity as a charge polarity of the biological material to the first electrode, and applying a voltage having an opposite polarity to the charge polarity to at least some of the second electrodes, the first electrode and the second electrodes each producing an electrostatic field by which the charged biological material is moved toward the second electrodes; and

a control device configured to control a process which is performed repeatedly, the process including

(a) supplying an intercalating agent onto the biological material detection element,

(b) measuring an electrochemical signal from the intercalating agent which is based on a reaction between the charged biological material and the ligand, and

(c) removing the intercalating agent adhering to the ligand.

20. The apparatus according to claim 19, wherein the driving circuit further performs a driving operation of applying a voltage having an opposite polarity to the charge polarity to some of the second electrodes in the circumferential direction and applying a voltage having the same polarity as the charge polarity to some other electrodes of the second electrodes, while sequentially changing positions of electrodes to which voltages having the opposite polarity and same polarity are to be applied.

21. The apparatus according to claim 19, wherein the biological material detection element further includes at least two annular electrodes concentrically arranged between the first electrode and the array of the second electrodes