SMALL VOLUME IN VITRO SENSOR AND METHODS OF MAKING
First Claim
1. A method of manufacturing electrochemical sensors, the method comprising steps of:
- (a) forming a plurality of working electrodes on a first substrate;
(b) forming a plurality of counter electrodes on a second substrate;
(c) forming a plurality of indicator electrodes on one of the first substrate and the second substrate;
(d) overlaying the first substrate and the second substrate to form a layered structure;
(e) creating a sample chamber region between the first substrate and the second substrate; and
(f) separating a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, at least one indicator electrode, and at least one sample chamber region.
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Accused Products
Abstract
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
223 Citations
11 Claims
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1. A method of manufacturing electrochemical sensors, the method comprising steps of:
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(a) forming a plurality of working electrodes on a first substrate;
(b) forming a plurality of counter electrodes on a second substrate;
(c) forming a plurality of indicator electrodes on one of the first substrate and the second substrate;
(d) overlaying the first substrate and the second substrate to form a layered structure;
(e) creating a sample chamber region between the first substrate and the second substrate; and
(f) separating a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, at least one indicator electrode, and at least one sample chamber region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification