System And Method For Cross-Talk Cancellation In A Multilane Fluorescence Detector
First Claim
1. A method for reducing cross-talk in a multi-channel data acquisition system, which system includes at least a multi-channel photodetector, comprising:
- determining a cross-talk matrix for the system;
operating the system to detect data, and applying the cross-talk matrix to the detected data to reduce or remove channel cross-talk in the detected data.
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Abstract
The present invention is directed to a system and method for cross-talk cancellation for multi-lane fluorescence detectors. The invention may be implemented in accordance with a variety of systems, including systems for multi-capillary electrophoresis. The present invention is based on a special calibration procedure for determination of a channel cross-talk matrix and enables an accurate separation of the fluorescence emitted from individual capillary lanes. The proposed method for cross-talk calibration and removal is very useful for design and development of multi-lane single photon counting detection systems.
42 Citations
33 Claims
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1. A method for reducing cross-talk in a multi-channel data acquisition system, which system includes at least a multi-channel photodetector, comprising:
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determining a cross-talk matrix for the system;
operating the system to detect data, and applying the cross-talk matrix to the detected data to reduce or remove channel cross-talk in the detected data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A system for reducing cross-talk in multi-channel data acquisition, comprising:
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a multi-channel photodetector;
a processor, and a memory coupled to the processor and containing instructions which, when executed by the processor, configure the processor to perform steps comprising;
determining a cross-talk matrix;
operating the system to detect data, and applying the cross-talk matrix to the detected data to reduce cross-talk in the detected data. - View Dependent Claims (14, 15, 16, 17)
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18. A system for multi-capillary data acquisition comprising;
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a light source;
at least one multi capillary array positioned to receive light from the light source and filled with a material which produces a useful radiation caused by illumination with said laser source;
a device for separating and identifying more than one spectral band of said useful radiation;
a multi channel photodetector positioned to receive said useful radiation from the multi capillary array;
a processor configured to receive data originating from the photodetector and a memory coupled to the processor and containing instructions which, when executed by the processor, configure the processor to perform steps comprising;
determining a cross-talk matrix;
operating the system to detect fluorescence data, and applying the cross-talk matrix to the fluorescence data to reduce cross-talk in the fluorescence data. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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30. A method for determining a cross-talk matrix for a multi-capillary data acquisition system, comprising:
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applying an input sample to a first capillary;
detecting fluorescence at a first channel which corresponds to the first capillary and at least a second channel;
recording the detected fluorescence data;
processing the recorded data from the first and the second channels to determine what fraction of the fluorescence from the input sample was received by the first channel and what fraction of the fluorescence from the input sample was received by the second channel, and recording the determined fractions in a column of the cross talk matrix. - View Dependent Claims (31, 32, 33)
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Specification