Positive Selection Procedure for Optically Directed Selection of Cells
First Claim
Patent Images
1. A method for positive cell selection comprising:
- providing a substrate with multiple locations;
immobilizing one or more cells comprising a photosensitive repair system and capable of exhibiting an imagable property when excited, indicating a desirable cell, either onto or within said substrate;
detecting the desirable cells by applying an excitation light source to the substrate to excite the imagable property of at least one of the one or more cells;
recording locations containing the desirable cells and locations not containing the desirable cells;
generating a repair image from the locations of the desirable cells;
applying a lethal light source to the substrate wherein the lethal light source is lethal to the cells, if the cells are not exposed to a repair light source capable of activating the photosensitive repair systems of the cells scanned; and
projecting a repair light source according to the repair image onto the substrate, the projection having light and dark portions wherein the light portions are capable of activating the photosensitive repair systems of the cells projected upon.
1 Assignment
0 Petitions
Accused Products
Abstract
Provided are methods and systems that can be used as a means of positive selection by initiating light mediated DNA repair in cells, having photosensitive repair systems, with desired characteristics from libraries of mutants that have been deactivated with a lethal light source.
48 Citations
30 Claims
-
1. A method for positive cell selection comprising:
-
providing a substrate with multiple locations;
immobilizing one or more cells comprising a photosensitive repair system and capable of exhibiting an imagable property when excited, indicating a desirable cell, either onto or within said substrate;
detecting the desirable cells by applying an excitation light source to the substrate to excite the imagable property of at least one of the one or more cells;
recording locations containing the desirable cells and locations not containing the desirable cells;
generating a repair image from the locations of the desirable cells;
applying a lethal light source to the substrate wherein the lethal light source is lethal to the cells, if the cells are not exposed to a repair light source capable of activating the photosensitive repair systems of the cells scanned; and
projecting a repair light source according to the repair image onto the substrate, the projection having light and dark portions wherein the light portions are capable of activating the photosensitive repair systems of the cells projected upon. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. A method for positive cell selection comprising:
-
providing a substrate with multiple locations;
immobilizing one or more cells comprising a photosensitive repair system and capable of exhibiting an imagable property when excited, indicating a desirable cell, either onto or within said substrate;
detecting the desirable cells by scanning a first light beam across the substrate to excite the imagable property of at least one of the one or more cells;
recording locations containing the desirable cells and locations not containing the desirable cells;
generating a repair image from the locations of the desirable cells;
scanning a second light beam across the substrate wherein the second light beam is lethal to the cells, if the cells are not exposed to a light beam capable of activating the photosensitive repair systems of the cells scanned; and
scanning a third light beam, according to the repair image, wherein the third light beam is capable of activating the photosensitive repair systems of the cells scanned. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
-
-
19. An apparatus for positive cell selection comprising:
-
a means for holding a sample of cells comprising a photosensitive repair system and capable of exhibiting an imagable property when excited, indicating a desirable cell;
an excitation light source capable of exciting the imagable property;
a means for detecting the location of the desirable cells;
a means for generating a repair image representative of the locations of the desirable cells;
a lethal light source capable of killing the sample of cells, if not exposed to a light source capable of activating the photosensitive repair systems of the cells illuminated; and
a repair light source capable of activating the photosensitive repair systems according to the repair image. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
-
Specification