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Method and system for the generation of large double stranded DNA fragments

  • US 20070196834A1
  • Filed: 08/17/2006
  • Published: 08/23/2007
  • Est. Priority Date: 09/09/2005
  • Status: Abandoned Application
First Claim
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1. A method for the generation of a long double stranded DNA target sequence comprising:

  • (a) synthesizing a set of oligonucleotides that contain sections of the target sequence, each oligonucleotide attached to a support by a cleavable linker;

    (b) cleaving the linker to release selected oligonucleotides in a desired sequence, bringing the released oligonucleotides together, and joining selected oligonucleotides to form a set of subsequences which are parts of the desired target sequence; and

    (c) assembling the subsequences to form the desired target sequence.

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