Reprogramming of adult human testicular stem cells to pluripotent germ-line stem cells

• US 20070196918A1
• Filed: 03/30/2007
• Published: 08/23/2007
• Est. Priority Date: 07/15/2004
• Status: Abandoned Application

• Current Assignee: PrimeGen Biotech LLC
• Original Assignee: PrimeGen Biotech LLC
• Inventors: Silva, Francisco, Sayre, Chauncey, Pau, Kwok-Yuen
• Application Number:
  US11/694,687
• Publication Number:
  US 20070196918A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C12N 2500/25 Insulin-transferrin; Insulin-transferrin-selenium
• CPC Class Codes: C12N 2500/32 Amino acids
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/119 Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
• CPC Class Codes: C12N 2501/13 Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2501/392 Sexual steroids
• CPC Class Codes: C12N 5/0611 Primordial germ cells, e.g. embryonic germ cells [EG]

Abstract

Methods for therapeutically programming human adult stem cells into pluripotent cells are provided. Cell therapeutically programmed from adult testicular cells are disclosed. The therapeutically reprogrammed cells are suitable for cellular regenerative therapy and have the potential to differentiate into more committed cell lineages.

Claims

1. A method for therapeutic reprogramming comprising:

isolating a human adult stem cell;

contacting said human adult stem cell with a medium comprising stimulatory factors which induce development of said stem cell into a therapeutically reprogrammed cell;

recovering said therapeutically reprogrammed cell from said medium.

2. The method of claim 1 wherein said human adult stem cell is isolated from the testes.

3. The method of claim 2 wherein said human adult stem cell is a spermatogonial stem cell.

4. The method of claim 1 wherein said medium comprises PM-10™

medium.

5. The method of claim 1 wherein said therapeutically reprogrammed cell is matured into a more terminally differentiated cell.

6. The method of claim 5 wherein said more terminally differentiated cell is a cardiac myocyte.

7. The method of claim 5 wherein said more terminally differentiated cell is a neural cell.

8. The method of claim 1 further comprising the step of culturing said therapeutically reprogrammed cell to form a cell line.

9. The method of either of claims 1 or 8 further comprising the step of implanting said therapeutically reprogrammed cell, or a cell matured therefrom, into a host in need of a therapeutically reprogrammed cell.

12. The pluripotent therapeutic composition of claim 10 wherein said therapeutically reprogrammed human adult stem cell is produced according to the therapeutic reprogramming method of claim 1.

10. A pluripotent therapeutic composition comprising a therapeutically reprogrammed human adult stem cell.

11. The pluripotent therapeutic composition of claim 10 wherein said human adult stem cell is isolated from the testes.

13. A pluripotent therapeutic composition comprising a therapeutically reprogrammed human adult stem cell which has been induced into a more terminally differentiated cell prior to implantation into a host in need of a therapeutically reprogrammed cells.