Method and apparatus for DNA purification
First Claim
1. A method for DNA purification, which comprises:
- contacting a silicone structure having a SiO2 layer formed on a surface thereof with a fluid sample containing DNA, wherein the fluid sample has a pH lower than a pKa of silanol;
removing the fluid sample from the silicone structure; and
treating the silicone structure with a buffer having a higher pH than the pKa of silanol.
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Accused Products
Abstract
A method and apparatus for DNA purification are provided which use a silicone structure having a Sio2 layer formed on its surface. In the method, DNA is effectively purified from a fluid sample by maintaining the pH of the silicone structure'"'"'s surface to be lower than the pKa of silanol to make the surface of the silicone structure charged with hydroxyl groups which results in the binding of DNA present in the fluid sample, removing the residual fluid sample which fails to bind to the surface of the silicone structure, and altering the pH of the silicone structure'"'"'s surface to be higher than the pKa of silanol to separate the bound DNA from the surface of the silicone structure.
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Citations
12 Claims
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1. A method for DNA purification, which comprises:
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contacting a silicone structure having a SiO2 layer formed on a surface thereof with a fluid sample containing DNA, wherein the fluid sample has a pH lower than a pKa of silanol;
removing the fluid sample from the silicone structure; and
treating the silicone structure with a buffer having a higher pH than the pKa of silanol. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An apparatus for DNA purifications, which comprises:
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a silicone structure having a SiO2 layer formed on a surface of the silicone structure, wherein the silicone structure comprises a definite space, an inlet for introducing a fluid into the definite space, and an outlet for discharging the fluid from the definite space;
a sample storage compartment for storing a fluid sample comprising DNA; and
a buffer storage compartment for storing a buffer having a higher pH than a pKa of silanol . - View Dependent Claims (8, 9, 10, 11, 12)
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Specification