Systems and methods of lipoprotein size fraction assaying
First Claim
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1. A nanopore analysis system, comprising:
- a nanopore flow cell comprising a first structure that separates two independent adjacent pools of a medium, a first nanopore aperture through the first structure, the first nanopore aperture dimensioned to allow fluid communication between the two independent adjacent pools, and an electrode adjacent and in electrical communication with the first nanopore aperture; and
a detection system designed to detect the size of the lipoproteins translocated through the first nanopore aperture.
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Abstract
Systems and methods for nanopore flow cells are provided.
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Citations
20 Claims
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1. A nanopore analysis system, comprising:
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a nanopore flow cell comprising a first structure that separates two independent adjacent pools of a medium, a first nanopore aperture through the first structure, the first nanopore aperture dimensioned to allow fluid communication between the two independent adjacent pools, and an electrode adjacent and in electrical communication with the first nanopore aperture; and
a detection system designed to detect the size of the lipoproteins translocated through the first nanopore aperture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for analyzing a lipoprotein, comprising:
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providing a nanopore analysis system;
introducing a target lipoprotein to a nanopore flow cell in the nanopore analysis system;
applying a voltage gradient to the nanopore analysis system;
translocating the target lipoprotein through a nanopore aperture in the nanopore analysis system; and
monitoring a signal corresponding to the movement of the target lipoprotein with respect to the nanopore aperture. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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18. An array for size fractioning of lipoproteins, the array comprising:
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a structure including a first layer fluidly coupled to a second layer, the first layer comprising a first surface exposed to a first pool of fluid into which a sample of lipoproteins can be introduced, and a second surface exposed to a second pool of fluid into which lipoproteins can be translocated;
a plurality of nanopores through the first layer;
electrodes associated with each nanopore; and
a second layer, wherein the first layer and the second layer form part of an enclosure for the second pool.
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19. An array for size fractioning of lipoproteins, the array comprising:
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a plurality of structures;
an associated membrane disposed in each structure; and
a plurality of nanopores disposed through each membrane, wherein each membrane comprises nanopores that are of a different size than nanopores of membrane(s) disposed adjacent it; and
wherein each nanopore is associated with a specific region, and wherein the regions of each membrane are aligned. - View Dependent Claims (20)
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Specification