Masked Solid Supports

• US 20070202013A1
• Filed: 04/20/2005
• Published: 08/30/2007
• Est. Priority Date: 04/21/2004
• Status: Abandoned Application

• Current Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Original Assignee: PamGene B.V. (VitrOmics Healthcare Holding BV)
• Inventors: Ingham, Colin
• Application Number:
  US10/593,793
• Publication Number:
  US 20070202013A1
• Time in Patent Office: Days
• US Class Current: 422/99
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00427 using masks
• CPC Class Codes: B01J 2219/00639 the compounds being trapped in or bound to a porous medium
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00664 Three-dimensional arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00743 Cells

Abstract

The present invention relates to methods for the manufacture of a masked solid support suitable for array analysis comprising the steps of: (i) providing a solid porous support suitable for array analysis having first and second surfaces, said solid porous support having channels extending from said first surface to said second surface; (ii) depositing at a predefined area on said porous solid support a polymeric material to said first surface, said polymeric material comprising a co-solvent so as to temporarily decrease the viscosity and/or rate of polymerisation of the polymeric material during the step of depositing; (iii) allowing said polymeric material to enter said channels of said solid porous support; (iv) removing said co-solvent by contacting said first surface with a wash solution and extracting the co-solvent/wash solution through said channels so as to restore the polymerisation rate of the polymer material within said channels, whereby a mask on said solid support is formed. The present invention further relates to solid supports manufactured by said methods and uses thereof.

Claims

1. A method for the manufacture of a masked solid support suitable for array analysis comprising the steps of:

(i) providing a solid porous support suitable for array analysis having first and second surfaces, said solid porous support having channels extending from said first surface to said second surface;

(ii) depositing at a predefined area on said porous solid support a polymeric material to said first surface, said polymeric material comprising a co-solvent so as to temporarily decrease the viscosity and/or rate of polymerization of the polymeric material during the step of depositing;

(iii) allowing said polymeric material to enter said channels of said solid porous support;

(iv) removing said co-solvent by contacting said first surface with a wash solution and extracting the co-solvent/wash solution through said channels so as to restore the polymerization rate of the polymer material within said channels, whereby a mask on said solid support is formed.

2. The method according to claim 1, wherein said co-solvent is chosen from the group comprising glycols and glycol ethers.

3. The method according to claim 1, wherein said mask forms a grid of polymeric material through said solid porous support.

4. The method according to claim 1, wherein said polymeric material comprises an agent, said agent affecting the mask properties.

5. The method according to claim 4, wherein said mask properties are chosen from the group comprising electricity conduction, colour, magnetical charge, hydrophobicity, adhesion/absorption of microorganisms, adhesion/adsorption by tissue culture cells, and repellent/attracting property.

6. The method according to claim 1, wherein said polymeric material is a latex polymer.

7. The method according to claim 1, wherein said depositing step is by a means chosen from the group comprising a high precision x-y-z pipettor, inkjet printer, and manual handling.

8. The method according claim 1, wherein said solid porous support is a flow-through support.

9. The method according to claim 1, wherein said solid porous support is a metal oxide support.

10. The method according to claim 9, wherein said metal oxide is aluminium oxide.

11. A masked solid porous support suitable for array analysis comprising first and second surfaces, said solid porous support having channels extending from said first surface to said second surface;

wherein at a predefined area on said porous solid support a polymeric material is present, wherein said polymeric material is within the channels and forms a mask on the solid porous support.

12. The solid porous support according to claim 11, wherein said mask forms a grid of polymeric material through said solid porous support.

13. The solid porous support according to claim 11, wherein said polymeric material comprises an agent, said agent affecting the mask properties.

14. The solid porous support according to claim 11, wherein said polymeric material is a latex polymer.

15. The solid porous support according to claim 11, wherein said solid porous support is a flow-through support.

16. The solid porous support according to claim 11, wherein said solid porous support is a metal oxide support.

21. A kit for array analysis comprising the it solid porous support according to claim 11.

17. The solid porous support according to claim 17, wherein said metal oxide is aluminium oxide.

18-20. -20. (canceled)